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Literature summary extracted from

  • Inouye, K.; Minoda, M.; Takita, T.; Sakurama, H.; Hashida, Y.; Kusano, M.; Yasukawa, K.
    Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization (2006), Protein Expr. Purif., 46, 248-255.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.27 0.4
-
 N-carbobenzoxy-L-Asp-L-Phe methyl ester pH 7.5, 25°C, recombinant enzyme Bacillus thermoproteolyticus
3.4.24.27 0.52
-
 N-carbobenzoxy-L-Asp-L-Phe methyl ester pH 7.5, 25°C, native enzyme Bacillus thermoproteolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.27 34600
-
 x * 34600, SDS-PAGE Bacillus thermoproteolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Bacillus thermoproteolyticus
-
 expressed in Escherichia coli
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.27 proteolytic modification synthesis as a pre-proprotein Bacillus thermoproteolyticus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.27 expression as single polypeptide pre-proenzyme in Escherichia coli, secretion into medium as mature enzyme Bacillus thermoproteolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.27 9500
-
 pH 7.5, 25°C Bacillus thermoproteolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 N-carbobenzoxy-L-Asp-L-Phe-methyl ester + H2O
-
 Bacillus thermoproteolyticus N-carbobenzoxy-L-Asp + L-Phe-methyl ester
-
 ?
3.4.24.27 N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide + H2O
-
 Bacillus thermoproteolyticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.27 ? x * 34600, SDS-PAGE Bacillus thermoproteolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.27 3.8
-
 N-carbobenzoxy-L-Asp-L-Phe methyl ester pH 7.5, 25°C, recombinant enzyme Bacillus thermoproteolyticus
3.4.24.27 4.3
-
 N-carbobenzoxy-L-Asp-L-Phe methyl ester pH 7.5, 25°C, native enzyme Bacillus thermoproteolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.27 6.5 7
-
 Bacillus thermoproteolyticus