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Literature summary extracted from
- Identification of a thermostable and enantioselective amidase from the thermoacidophilic archaeon Sulfolobus tokodaii strain 7 (2006), Protein Expr. Purif., 45, 368-373.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | gene ST0478, DNA and amino acid sequence determination and analyis, expression in Escherichia coli strain BL21(DE3) as insoluble aggregated His6-tagged protein | Sulfurisphaera tokodaii |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 47000 | - |
x * 47000, recombinant His6-tagged enzyme, SDS-PAGE | Sulfurisphaera tokodaii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.4 | Sulfurisphaera tokodaii | - |
gene ST0478 | - |
| 3.5.1.4 | Sulfurisphaera tokodaii 7 | - |
gene ST0478 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | recombinant insoluble aggregated His6-tagged protein from Escherichia coli by denaturation, refolding during nickel affinity chromatography, gel filtration, and heat treatment, 2.9fold to homogeneity | Sulfurisphaera tokodaii |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | denaturation and refolding during nickel affinity chromatography of the recombinant insoluble aggregated His6-tagged protein from Escherichia coli | Sulfurisphaera tokodaii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 14.8 | - |
purified recombinant His6-tagged enzyme | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.4 | (R,S)-2-phenylpropionamide + H2O | enantioselective reaction with preference for the S-enantiomer | Sulfurisphaera tokodaii | (S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3 | - |
? |  |
| 3.5.1.4 | (R,S)-2-phenylpropionamide + H2O | enantioselective reaction with preference for the S-enantiomer | Sulfurisphaera tokodaii 7 | (S)-2-phenylpropionic acid + (R)-2-phenylpropionamide + NH3 | - |
? | |
| 3.5.1.4 | benzamide + H2O | - |
Sulfurisphaera tokodaii | benzoic acid + NH3 | - |
? | |
| 3.5.1.4 | benzamide + H2O | - |
Sulfurisphaera tokodaii 7 | benzoic acid + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.4 | ? | x * 47000, recombinant His6-tagged enzyme, SDS-PAGE | Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 75 | - |
about, recombinant enzyme | Sulfurisphaera tokodaii |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 37 | 80 | 25% of maximal activity at 37°C, maximal activity at 75°C, about 90% of maximal activity at 80°C | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | additional information | - |
the purified recombinant enzyme is highly thermostable | Sulfurisphaera tokodaii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 7 | 8 | recombinant enzyme | Sulfurisphaera tokodaii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 3 | 9 | 15% of maximal activity at pH 3.0, 65% at pH 9.0 | Sulfurisphaera tokodaii |
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