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Literature summary extracted from
- Cloning, expression, and catalytic triad of recombinant arylformamidase (2005), Protein Expr. Purif., 44, 39-44.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.9 | DNA and amino acid sequence determination and anlysis, expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH10B | Mus musculus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.1.9 | D247A | site-directed mutagenesis, inactive mutant | Mus musculus |
| 3.5.1.9 | H279A | site-directed mutagenesis, inactive mutant | Mus musculus |
| 3.5.1.9 | S162A | site-directed mutagenesis, inactive mutant | Mus musculus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.9 | 0.18 | - |
N-formyl-L-kynurenine | pH 7.4, native wild-type enzyme | Mus musculus |  |
| 3.5.1.9 | 0.19 | - |
N-formyl-L-kynurenine | pH 7.4, recombinant wild-type enzyme | Mus musculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.9 | cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.9 | 34229 | - |
x * 34229, sequence calculation | Mus musculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.9 | N-formyl-L-kynurenine + H2O | Mus musculus | - |
formate + L-kynurenine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.9 | Mus musculus | Q8K4H1 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.9 | native enzyme from liver cytosol, recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography, gel filtration, and nickel affinity chromatography, to high purity, recombinant wild-type enzyme 90fold | Mus musculus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.5.1.9 | N-formyl-L-kynurenine + H2O = formate + L-kynurenine | the catalytic triad is formed by residues Ser162, Asp247, and His279 | Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.9 | liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.9 | 42 | - |
purified recombinant wild-type enzyme | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.9 | N-formyl-L-kynurenine + H2O | - |
Mus musculus | formate + L-kynurenine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.9 | ? | x * 34229, sequence calculation | Mus musculus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.9 | AFMID | - |
Mus musculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.9 | 7.4 | - |
assay at | Mus musculus |
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