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Literature summary extracted from
- In vitro refolding of carboxypeptidase T precursor from Thermoactinomyces vulgaris obtained in Escherichia coli as cytoplasmic inclusion bodies (2005), Protein Expr. Purif., 40, 51-59.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.18 | Subtilisin | cleavage of the carboxypeptidase precursor | Thermoactinomyces vulgaris |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.17.18 | expression in Escherichia coli | Thermoactinomyces vulgaris |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.17.18 | Ca2+ important for thermostability | Thermoactinomyces vulgaris |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.18 | Ca2+ | 10 mM, required for enzyme stabilization | Thermoactinomyces vulgaris |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.18 | 38000 | - |
SDS-PAGE, mature enzyme | Thermoactinomyces vulgaris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.18 | Thermoactinomyces vulgaris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.18 | purification from inclusion bodes | Thermoactinomyces vulgaris |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.4.17.18 | best conditions: 30 mM Tris-HCl pH 9.0, 0.5 M NaCl, 30% glycerol, 2 mM dithiothreitol, 2 mM cysteine, 10 mM CaCl2, 40°C for 12 h | Thermoactinomyces vulgaris |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.17.18 | cell culture | - |
Thermoactinomyces vulgaris | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.18 | 8.2 | - |
0.2 ml 50 mM Tris-HCl pH 8.0, 5 mM CaCl2, 0.2 ml 2,4-dinitrophenyl-Ala-Ala-Arg, 37°C | Thermoactinomyces vulgaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.18 | 2,4-dinitrophenyl-Ala-Ala-Arg + H2O | - |
Thermoactinomyces vulgaris | 2,4-dinitrophenyl-Ala-Ala + Arg | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.17.18 | monomer | 1 * 38000, SDS-PAGE, mature enzyme | Thermoactinomyces vulgaris |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.17.18 | carboxypeptidase T | - |
Thermoactinomyces vulgaris |
| 3.4.17.18 | CPT | - |
Thermoactinomyces vulgaris |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.18 | 65 | - |
10% denatured protein after 2 h at 65°C, 30 mM Tris-HCl pH 9.0, 0.5 M NaCl, 30% glycerol, 2 mM dithiothreitol, 2 mM cysteine, 10 mM CaCl2, rapid denaturing occurs without CaCl2 under the same conditions | Thermoactinomyces vulgaris |
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Release 2023.1 (January 2023)
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