Literature summary extracted from

Chen, H.; Lin, K.; Lu, C.
Refolding and activation of recombinant N-carbamoyl-D-amino acid amidohydrolase from Escherichia coli inclusion bodies (2005), Process Biochem., 40, 2135-2141.

No PubMed abstract available

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.77	Agrobacterium tumefaciens	-	plasmid cloned in Escherichia coli	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.77	denatured recombinant enzyme	Agrobacterium tumefaciens

Renatured (Commentary)

EC Number	Renatured (Comment)	Organism
3.5.1.77	refolding of purified denatured DCaseH is best yielded at 50% glycerol for 3 days with a 20% renaturation yield. Approximately 38% of the native secondary structure is reformed. The refolding with the addition of glycerol partially recovers both the activity and the native-like secondary structure of the denatured enzyme	Agrobacterium tumefaciens

Synonyms

EC Number	Synonyms	Comment	Organism
3.5.1.77	N-carbamoyl-D-amino acid amidohydrolase	-	Agrobacterium tumefaciens

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Release 2023.1 (January 2023)