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Literature summary extracted from

  • Sun, D.; McNicol, A.; James, A.A.; Peng, Z.
    Expression of functional recombinant mosquito salivary apyrase: a potential therapeutic platelet aggregation inhibitor (2006), Platelets, 17, 178-184.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.6.1.5 medicine enzyme inhibits ADP- collagen- and thrombin-induced human platelet aggregation in dose-dependent manner, use as a therapeutic agent for inhibition of platelet-mediated thrombosis Aedes aegypti
3.6.1.5 pharmacology the enzyme may serve as a therapeutic agent for inhibition of platelet-mediated thrombosis Aedes aegypti

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.5 expression in Spodoptera frugiperda Sf9 cells using the baculovirus infection system Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.5 0.0125
-
 ADP
-
 Aedes aegypti
3.6.1.5 0.0125
-
 ADP pH 7.5, 37°C Aedes aegypti
3.6.1.5 0.015
-
 ATP
-
 Aedes aegypti
3.6.1.5 0.015
-
 ATP pH 7.5, 37°C Aedes aegypti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.5 extracellular
-
 Aedes aegypti
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.5 Ca2+
-
 Aedes aegypti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.5 68000
-
 x * 68000, SDS-PAGE Aedes aegypti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.5 ADP + H2O Aedes aegypti
-
 AMP + phosphate
-
 ?
3.6.1.5 ATP + H2O Aedes aegypti
-
 ADP + phosphate
-
 ?
3.6.1.5 additional information Aedes aegypti enzyme inhibits ADP-, collagen-, and thrombin-induced human platelet aggregation in dose-dependent manner ?
-
 ?
3.6.1.5 additional information Aedes aegypti apyrase, an ecto-enzyme with ADPase and ATPase activities, rapidly metabolizes ADP and ATP released from platelets and endothelial cells, thereby reducing platelet activation and recruitment. The recombinant apyrase inhibits ADP-, collagen- and thrombin-induced human platelet aggregation, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.5 Aedes aegypti
-
-
-
3.6.1.5 Aedes aegypti
-
 recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.5 recombinant enzyme from insect Sf9 cells by anion exchange chromatography to homogeneity, large scale preparation Aedes aegypti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.5 additional information apyrase is an ecto-enzyme Aedes aegypti
-
3.6.1.5 saliva
-
 Aedes aegypti
-
3.6.1.5 salivary gland enzyme synthesis Aedes aegypti
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.5 ADP + H2O
-
 Aedes aegypti AMP + phosphate
-
 ?
3.6.1.5 ATP + 2 H2O
-
 Aedes aegypti AMP + 2 phosphate
-
 ?
3.6.1.5 ATP + H2O
-
 Aedes aegypti ADP + phosphate
-
 ?
3.6.1.5 additional information enzyme inhibits ADP-, collagen-, and thrombin-induced human platelet aggregation in dose-dependent manner Aedes aegypti ?
-
 ?
3.6.1.5 additional information apyrase, an ecto-enzyme with ADPase and ATPase activities, rapidly metabolizes ADP and ATP released from platelets and endothelial cells, thereby reducing platelet activation and recruitment. The recombinant apyrase inhibits ADP-, collagen- and thrombin-induced human platelet aggregation, overview Aedes aegypti ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.5 ? x * 68000, SDS-PAGE Aedes aegypti

Synonyms

EC Number Synonyms Comment Organism
3.6.1.5 Apyrase
-
 Aedes aegypti
3.6.1.5 ATP diphosphohyrolase
-
 Aedes aegypti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.5 37
-
 assay at Aedes aegypti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.5 7.5
-
 assay at Aedes aegypti