EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.27 | Mg2+ | dependent on | Populus tremuloides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.27 | dimethylallyl diphosphate | Populus tremuloides | - |
isoprene + diphosphate | - |
ir | |
4.2.3.27 | additional information | Populus tremuloides | the acid-catalyzed non-enzymatic conversion of dimethylallyl diphosphate is negligible at physiologic pH | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.27 | Populus tremuloides | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.27 | native enzyme partially from leaves by ammonium sulfate fractionation | Populus tremuloides |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.3.27 | leaf | - |
Populus tremuloides | - |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.3.27 | -70°C, partially purified enzyme, 40-55% ammonium sulfate fraction, in phosphate buffered saline, pH 7.4, 1mM DTT, stable for at least 1 month | Populus tremuloides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.27 | dimethylallyl diphosphate | - |
Populus tremuloides | isoprene + diphosphate | - |
ir | |
4.2.3.27 | dimethylallyl diphosphate | - |
Populus tremuloides | isoprene + diphosphate | i.e. 2-methyl-1,3-butadiene, volatile product, product determination by gas chromatography | ir | |
4.2.3.27 | additional information | the acid-catalyzed non-enzymatic conversion of dimethylallyl diphosphate is negligible at physiologic pH | Populus tremuloides | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.3.27 | 28 | - |
assay at | Populus tremuloides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.27 | 7 | 9 | - |
Populus tremuloides |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.3.27 | 5.5 | 10 | - |
Populus tremuloides |