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Literature summary extracted from

  • Murata, J.; De Luca, V.
    localization of tabersonine 16-hydroxylase and 16-OH tabersonine-16-O-methyltransferase to leaf epidermal cells defines them as a major site of precursor biosynthesis in the vindoline pathway in Catharanthus roseus (2005), Plant J., 44, 581-594.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.19.62 additional information involved in vindoline biosynthesis Catharanthus roseus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.103 T16H expression analysis in leaves, overview, recombinant expression in Escherichia coli Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.103 additional information Catharanthus roseus the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview ?
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 ?
1.14.14.103 tabersonine + NADPH + H+ + O2 Catharanthus roseus
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 16-hydroxytabersonine + NADP+ + H2O
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.103 Catharanthus roseus
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-
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1.14.19.62 Catharanthus roseus
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-
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3.2.1.105 Catharanthus roseus
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.103 epidermal cell predominantly Catharanthus roseus
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1.14.14.103 leaf quantitative expression analysis in leaves Catharanthus roseus
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1.14.14.103 additional information localization study Catharanthus roseus
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1.14.19.62 epidermal cell leaf epidermal cells are biosynthetically competent to produce secologanin precursors that are converted via many enzymatic transformations to make 16-methoxytabersonine, which is then transported to cells within Catharanthus leaves to complete the last three or four enzymatic transformations to make vindoline Catharanthus roseus
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1.14.19.62 leaf
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 Catharanthus roseus
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3.2.1.105 leaf epidermis abaxial and adaxial Catharanthus roseus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.103 additional information the enzyme is involved in biosynthesis of precursors in the vindoline pathway in Catharanthus roseus leaf epidermal cells, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview Catharanthus roseus ?
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 ?
1.14.14.103 tabersonine + NADPH + H+ + O2
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 Catharanthus roseus 16-hydroxytabersonine + NADP+ + H2O
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 ?
3.2.1.105 strictosidine + H2O The structural diversity of monoterpenoid indole alkaloids comes first from the deglucosylation of strictosidine by strictosidine beta-glucosidase. The unstable and versatile aglycone is biochemically converted by mostly uncharacterized enzyme reactions to the Corynanthe, Iboga and Aspidosperma classes of alkaloids such as ajmalicine, catharanthine and tabersonine, respectively Catharanthus roseus D-glucose + strictosidine aglycone
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 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.103 T16H
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 Catharanthus roseus
3.2.1.105 SGD
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 Catharanthus roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.103 37
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 assay at Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.103 cytochrome P450
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 Catharanthus roseus