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Literature summary extracted from

  • Waldhauser, S.S.M.; Gillies, F.M.; Crozier, A.; Baumann, T.W.
    Separation of the N-7 methyltransferase, the key enzyme in caffeine biosynthesis (1997), Phytochemistry, 45, 1407-1414.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.158 Mg2+
-
 Coffea arabica
2.1.1.159 Mg2+
-
 Coffea arabica
2.1.1.160 Mg2+
-
 Coffea arabica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.158 40000
-
 2 * 40000, SDS-PAGE Coffea arabica
2.1.1.158 67000
-
 about, gel filtration Coffea arabica
2.1.1.159 67000
-
 about, gel filtration Coffea arabica
2.1.1.160 40000
-
 2 * 40000, SDS-PAGE Coffea arabica
2.1.1.160 67000
-
 about, gel filtration Coffea arabica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine Coffea arabica first step in caffeine biosynthesis pathway from xanthosine S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine Coffea arabica second step in caffeine biosynthesis pathway S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine Coffea arabica last step in caffeine biosynthesis pathway S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.158 Coffea arabica
-
-
-
2.1.1.159 Coffea arabica
-
-
-
2.1.1.160 Coffea arabica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.158 native enzyme 9fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration Coffea arabica
2.1.1.159 native enzyme 39fold from young leaves by ammonium sulfate fractionation, 3 steps of ion exchange chromatography, and gel filtration Coffea arabica
2.1.1.160 native enzyme 24fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration Coffea arabica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.158 leaf young Coffea arabica
-
2.1.1.159 leaf young Coffea arabica
-
2.1.1.160 leaf young Coffea arabica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.158 0.32
-
 purified enzyme Coffea arabica
2.1.1.159 15.45
-
 purified enzyme Coffea arabica
2.1.1.160 3.65
-
 purified enzyme Coffea arabica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine
-
 Coffea arabica S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ?
2.1.1.158 S-adenosyl-L-methionine + xanthosine first step in caffeine biosynthesis pathway from xanthosine Coffea arabica S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine second step in caffeine biosynthesis pathway Coffea arabica S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine last step in caffeine biosynthesis pathway Coffea arabica S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?

Subunits

EC Number Subunits Comment Organism
2.1.1.158 dimer 2 * 40000, SDS-PAGE Coffea arabica
2.1.1.159 dimer 2 * 40000-49000, SDS-PAGE Coffea arabica
2.1.1.160 dimer 2 * 40000, SDS-PAGE Coffea arabica

Synonyms

EC Number Synonyms Comment Organism
2.1.1.158 7-N-methyltransferase
-
 Coffea arabica
2.1.1.158 7NMT
-
 Coffea arabica
2.1.1.159 3NMT
-
 Coffea arabica
2.1.1.159 N-3 methyltransferase
-
 Coffea arabica
2.1.1.160 1NMT
-
 Coffea arabica
2.1.1.160 N-1 methyltransferase
-
 Coffea arabica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.158 30
-
 assay at Coffea arabica
2.1.1.159 30
-
 assay at Coffea arabica
2.1.1.160 30
-
 assay at Coffea arabica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.158 7.5
-
 assay at Coffea arabica
2.1.1.159 7.5
-
 assay at Coffea arabica
2.1.1.160 7.5
-
 assay at Coffea arabica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.158 Coffea arabica chromatofocusing 5.2 4.7
2.1.1.159 Coffea arabica chromatofocusing 5.1 4.5
2.1.1.160 Coffea arabica chromatofocusing 4.9 4