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Literature summary extracted from

  • Oliver, E.M.; Skuce, P.J.; McNair, C.M.; Knox, D.P.
    Identification and characterization of an asparaginyl proteinase (legumain) from the parasitic nematode, Haemonchus contortus (2006), Parasitology, 133, 237-244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.34 gene leg-1, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, expression in Escherichia coli as GST-fusion protein Haemonchus contortus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 1,10-phenanthroline 27.5% inhibition at 5 mM Haemonchus contortus
3.4.22.34 4-(2-aminoethyl) benzenesulfonyl fluoride AEBSF, 45.8% inhibition at 5 mM Haemonchus contortus
3.4.22.34 E64 17.1% inhibition at 0.001 mM Haemonchus contortus
3.4.22.34 leupeptin 47.3% inhibition at 1 mM Haemonchus contortus
3.4.22.34 N-ethylmaleimide complete inhibition at 2 mM Haemonchus contortus
3.4.22.34 pepstatin A 8.4% inhibition at 0.1 mM Haemonchus contortus
3.4.22.34 Z-Phe-Ala-CHN2 i.e. benzyloxycarbonyl-phenylalanyl-alanyl-diazomethane, 40.8% inhibition at 0.01 mM Haemonchus contortus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.34 membrane bound Haemonchus contortus 16020
-
3.4.22.34 microvillus surface, of intestinal cells Haemonchus contortus 5902
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.34 49000
-
 x * 49000, about, amino acid sequence calculation, x * 75000, recombinant GST-fusion enzyme, SDS-PAGE Haemonchus contortus
3.4.22.34 75000
-
 x * 49000, about, amino acid sequence calculation, x * 75000, recombinant GST-fusion enzyme, SDS-PAGE Haemonchus contortus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Haemonchus contortus Q2UVF3 gene leg-1; barber pole worm, parasitic nematode
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.34 intestine microvillar surface Haemonchus contortus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.34 1.35
-
 membrane-bound fraction Haemonchus contortus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
 Haemonchus contortus Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.34 ? x * 49000, about, amino acid sequence calculation, x * 75000, recombinant GST-fusion enzyme, SDS-PAGE Haemonchus contortus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 asparaginyl proteinase
-
 Haemonchus contortus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 7
-
-
 Haemonchus contortus