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Literature summary extracted from

  • Alseth, I.; Rognes, T.; Lindback, T.; Solberg, I.; Robertsen, K.; Kristiansen, K.I.; Mainieri, D.; Lillehagen, L.; Kolsto, A.B.; Bjoras, M.
    A new protein superfamily includes two novel 3-methyladenine DNA glycosylases from Bacillus cereus, AlkC and AlkD (2006), Mol. Microbiol., 59, 1602-1609.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.20 expression in Escherichia coli BL2, AlkC Bacillus cereus
3.2.2.21 expression in Escherichia coli BL21, AlkD Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.20 28000
-
 x * 28000, AlkC, SDS-PAGE Bacillus cereus
3.2.2.21 25000
-
 x * 25000, AlkD, SDS-PAGE Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.20 additional information Bacillus cereus AlkC is involved exclusively in the repair of alkylation damage ?
-
 ?
3.2.2.21 additional information Bacillus cereus AlkD is involved exclusively in the repair of alkylation damage ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.20 Bacillus cereus
-
-
-
3.2.2.21 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.20
-
 Bacillus cereus
3.2.2.21
-
 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.20 alkylated DNA + H2O AlkC is specific for 3-methylpurines Bacillus cereus 3-methyladenine + 3-methylguanine + ?
-
 ?
3.2.2.20 additional information AlkC is involved exclusively in the repair of alkylation damage Bacillus cereus ?
-
 ?
3.2.2.20 additional information no detectable affinity for hypoxanthine, 8-oxoguanine and 5-formyluracil Bacillus cereus ?
-
 ?
3.2.2.21 alkylated DNA + H2O AlkD has high activity towards 7-methylguanine but removes 3-methylguanine more slowly as compared with Escherichia coli AlkA Bacillus cereus 3-methyladenine + 7-methylguanine + 3-methylguanine
-
 ?
3.2.2.21 additional information AlkD is involved exclusively in the repair of alkylation damage Bacillus cereus ?
-
 ?
3.2.2.21 additional information no detectable affinity for hypoxanthine, 1,N6-ethenoadenine, 8-oxoguanine and 5-formyluracil Bacillus cereus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.20 ? x * 28000, AlkC, SDS-PAGE Bacillus cereus
3.2.2.21 ? x * 25000, AlkD, SDS-PAGE Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
3.2.2.20 AlkC
-
 Bacillus cereus
3.2.2.21 AlkD
-
 Bacillus cereus