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Literature summary extracted from
- Serine proteases from two cell types target different components of a complex that governs regulated intramembrane proteolysis of pro-sigmaK during Bacillus subtilis development (2005), Mol. Microbiol., 58, 835-846.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.B49 | expressed as His-tag fusion protein in Escherichia coli | Bacillus subtilis |
| 3.4.21.116 | expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Bacillus subtilis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.21.B49 | S309A | inactive | Bacillus subtilis |
| 3.4.21.116 | S378A | unable to process self cleavage, unable to trigger the activation of pro-sigmaK processing | Bacillus subtilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.B23 | BofA | inhibition possibly by blocking the active site and providing a metal ligand | Bacillus subtilis | |
| 3.4.24.B23 | additional information | inactive when bound to SpoIVFA and BofA, both proteins required for complete inhibition | Bacillus subtilis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.B49 | additional information | secreted by the mother cell into the intermembrane space between the forespore and the mother cell membrane | Bacillus subtilis | - |
- |
| 3.4.21.116 | endospore-forming forespore | secreted by the forespore into the intermembrane space between the forespore and the mother cell membrane | Bacillus subtilis | 42601 | - |
| 3.4.24.B23 | membrane | - |
Bacillus subtilis | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.B23 | additional information | metalloprotease | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B49 | BofA + H2O | Bacillus subtilis | involved in sigmaK signaling, acts in cooperation with SpoIVB, substrate and enzyme coexpressed in Escherichia coli, cleavage site near the C-terminus | fragments of BofA | - |
? |  |
| 3.4.21.B49 | additional information | Bacillus subtilis | autoproteolysis as observed for SpoIVB | ? | - |
? | |
| 3.4.21.B49 | additional information | Bacillus subtilis | no effect on SpoIVFA when coexpressed in Escherichia coli | ? | - |
? | |
| 3.4.21.B49 | additional information | Bacillus subtilis PV79 | autoproteolysis as observed for SpoIVB | ? | - |
? | |
| 3.4.21.B49 | additional information | Bacillus subtilis PV79 | no effect on SpoIVFA when coexpressed in Escherichia coli | ? | - |
? | |
| 3.4.21.B49 | additional information | Bacillus subtilis PY79 | autoproteolysis as observed for SpoIVB | ? | - |
? | |
| 3.4.21.B49 | additional information | Bacillus subtilis PY79 | no effect on SpoIVFA when coexpressed in Escherichia coli | ? | - |
? | |
| 3.4.21.116 | additional information | Bacillus subtilis | BofA is unsuitable as a substrate | ? | - |
? | |
| 3.4.21.116 | additional information | Bacillus subtilis | self cleavage | ? | - |
? | |
| 3.4.21.116 | additional information | Bacillus subtilis PV79 | BofA is unsuitable as a substrate | ? | - |
? | |
| 3.4.21.116 | additional information | Bacillus subtilis PV79 | self cleavage | ? | - |
? | |
| 3.4.21.116 | additional information | Bacillus subtilis PY79 | BofA is unsuitable as a substrate | ? | - |
? | |
| 3.4.21.116 | additional information | Bacillus subtilis PY79 | self cleavage | ? | - |
? | |
| 3.4.21.116 | SpoIVFA + H2O | Bacillus subtilis | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? | |
| 3.4.21.116 | SpoIVFA + H2O | Bacillus subtilis PV79 | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? | |
| 3.4.21.116 | SpoIVFA + H2O | Bacillus subtilis PY79 | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? | |
| 3.4.24.B23 | pro-sigmaK + H2O | Bacillus subtilis | involved in the sporulation process | sigmaK + peptide | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.B49 | Bacillus subtilis | - |
- |
- |
| 3.4.21.B49 | Bacillus subtilis PY79 | - |
- |
- |
| 3.4.21.116 | Bacillus subtilis | - |
- |
- |
| 3.4.21.116 | Bacillus subtilis PY79 | - |
- |
- |
| 3.4.24.B23 | Bacillus subtilis | - |
- |
- |
| 3.4.24.B23 | Bacillus subtilis PV79 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B49 | BofA + H2O | involved in sigmaK signaling, acts in cooperation with SpoIVB, substrate and enzyme coexpressed in Escherichia coli, cleavage site near the C-terminus | Bacillus subtilis | fragments of BofA | - |
? | |
| 3.4.21.B49 | GFP-delta27 BofA + H2O | - |
Bacillus subtilis | ? | one or two low molecular weight species detected | ? | |
| 3.4.21.B49 | additional information | autoproteolysis as observed for SpoIVB | Bacillus subtilis | ? | - |
? | |
| 3.4.21.B49 | additional information | no effect on SpoIVFA when coexpressed in Escherichia coli | Bacillus subtilis | ? | - |
? | |
| 3.4.21.B49 | additional information | autoproteolysis as observed for SpoIVB | Bacillus subtilis PV79 | ? | - |
? | |
| 3.4.21.B49 | additional information | no effect on SpoIVFA when coexpressed in Escherichia coli | Bacillus subtilis PV79 | ? | - |
? | |
| 3.4.21.B49 | additional information | autoproteolysis as observed for SpoIVB | Bacillus subtilis PY79 | ? | - |
? | |
| 3.4.21.B49 | additional information | no effect on SpoIVFA when coexpressed in Escherichia coli | Bacillus subtilis PY79 | ? | - |
? | |
| 3.4.21.116 | additional information | BofA is unsuitable as a substrate | Bacillus subtilis | ? | - |
? | |
| 3.4.21.116 | additional information | self cleavage | Bacillus subtilis | ? | - |
? | |
| 3.4.21.116 | additional information | BofA is unsuitable as a substrate | Bacillus subtilis PV79 | ? | - |
? | |
| 3.4.21.116 | additional information | self cleavage | Bacillus subtilis PV79 | ? | - |
? | |
| 3.4.21.116 | additional information | BofA is unsuitable as a substrate | Bacillus subtilis PY79 | ? | - |
? | |
| 3.4.21.116 | additional information | self cleavage | Bacillus subtilis PY79 | ? | - |
? | |
| 3.4.21.116 | SpoIVFA + H2O | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | Bacillus subtilis | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? | |
| 3.4.21.116 | SpoIVFA + H2O | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | Bacillus subtilis PV79 | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? | |
| 3.4.21.116 | SpoIVFA + H2O | involved in the regulation of sporulation, cleavage of the extracellular domain, essential for sigmaK processing, cleavage of SpoIVFA activates SpoIVFB which is the enzyme for pro-sigmaK processing | Bacillus subtilis PY79 | cleaved SpoIVFA + extracellular domain peptide of SpoIVFA | - |
? | |
| 3.4.24.B23 | pro-sigmaK + H2O | involved in the sporulation process | Bacillus subtilis | sigmaK + peptide | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.B49 | CtpB | PDZ-containing serine protease with similar organization as SpoIVB | Bacillus subtilis |
| 3.4.21.116 | SpoIVB | - |
Bacillus subtilis |
| 3.4.24.B23 | SPOIVFB | member of the site-2 protease family of the intramembrane-cleaving proteases | Bacillus subtilis |
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