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Literature summary extracted from

  • Ponnuraj, K.; Xu, Y.; Macon, K.; Moore, D.; Volanakis, J.E.; Narayana, S.V.
    Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase (2004), Mol. Cell, 14, 17-28.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.47 engineered subunit Bb with Cys-resiudes at positions 435 and 428, in complex with inhibitors 6-amidino-2-naphthyl-4-guanidinobenzoate or diisopropyl phosphate Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.47 additional information introduction of Cys-residues to form a disulfide bond at positions 428 and 435 of von Willebrandt factor type A domain of subunit Bb. Adaption of an active conformation by the domain, which is not sufficient to activate the enzyme catalytic apparatus Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.47 6-amidino-2-naphthyl-4-guanidinobenzoate
-
 Homo sapiens
3.4.21.47 diisopropyl phosphate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.47 2.75
-
 t-butyloxycarbonyl-Gly-L-Leu-L-Ala-L-Arg-thiobenzyl ester subunit Bb, mutant with Cys-residues at positions 428, 435 Homo sapiens
3.4.21.47 5.63
-
 t-butyloxycarbonyl-Gly-L-Leu-L-Ala-L-Arg-thiobenzyl ester subunit Bb, wild-type Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.47 Homo sapiens P00751
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.47 t-butyloxycarbonyl-Gly-L-Leu-L-Ala-L-Arg-thiobenzyl ester substrate of enzyme subunit Bb Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.47 0.28
-
 t-butyloxycarbonyl-Gly-L-Leu-L-Ala-L-Arg-thiobenzyl ester subunit Bb, mutant with Cys-residues at positions 428, 435 Homo sapiens
3.4.21.47 0.65
-
 t-butyloxycarbonyl-Gly-L-Leu-L-Ala-L-Arg-thiobenzyl ester subunit Bb, wild-type Homo sapiens