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Literature summary extracted from

  • Robles Lopez, S.M.; Hortua Triana, M.A.; Zimmermann, B.H.
    Cloning and preliminary characterization of the dihydroorotase from Toxoplasma gondii (2006), Mol. Biochem. Parasitol., 148, 93-98.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.3 expressed in Escherichia coli strain X7014 Toxoplasma gondii
3.5.2.3 expression in Escherichia coli mutant strain SØ1263 and strain X7014 Toxoplasma gondii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.3 0.063
-
 dihydroorotate purified recombinant enzyme, at 37°C Toxoplasma gondii
3.5.2.3 0.0643
-
 L-dihydroorotate purified recombinant protein Toxoplasma gondii
3.5.2.3 0.323
-
 N-carbamoyl-L-aspartate purified recombinant protein Toxoplasma gondii
3.5.2.3 0.323
-
 N-(aminocarbonyl)-L-aspartic acid purified recombinant enzyme, at 37°C Toxoplasma gondii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.3 Zn2+
-
 Toxoplasma gondii
3.5.2.3 Zn2+ contains 3.33 mol zinc per mol protein Toxoplasma gondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.3 44200
-
 estimated from sequence of cDNA Toxoplasma gondii
3.5.2.3 45600
-
 gel filtration, laser light scattering Toxoplasma gondii
3.5.2.3 45600
-
 gel filtration and laser light scattering Toxoplasma gondii

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.3 Arabidopsis thaliana O04904
-
-
3.5.2.3 Escherichia coli P05020
-
-
3.5.2.3 Homo sapiens P27708
-
-
3.5.2.3 Mesocricetus auratus P08955
-
-
3.5.2.3 Mycobacterium leprae
-
-
-
3.5.2.3 Plasmodium berghei A0A509AX79
-
-
3.5.2.3 Saccharomyces cerevisiae
-
-
-
3.5.2.3 Toxoplasma gondii Q8MXA5 strain RH lambda Zap II cDNA library, strain PC117 and 5TN
-
3.5.2.3 Toxoplasma gondii Q8MXA5 strains RH, PC117, and 5TN
-
3.5.2.3 Trypanosoma cruzi O76138
-
-
3.5.2.3 Ustilago maydis P31301
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.3 DEAE-Sephacel column chromatography Toxoplasma gondii
3.5.2.3 recombinant protein purified to homogeneity Toxoplasma gondii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.3 tachyzoite
-
 Toxoplasma gondii
-
3.5.2.3 tachyzoite maintained by serial passage in the peritoneal cavity of CFW mice Toxoplasma gondii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.3 18.3
-
 in the biosynthetic direction Toxoplasma gondii
3.5.2.3 18.33
-
 purified recombinant enzyme Toxoplasma gondii
3.5.2.3 18.4
-
 in the degradative direction Toxoplasma gondii

Storage Stability

EC Number Storage Stability Organism
3.5.2.3 -70°C, HEPES buffer, pH 7.4, 0.5% glycerol Toxoplasma gondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.3 dihydroorotate + H2O
-
 Toxoplasma gondii N-carbamoyl-L-aspartate
-
 r
3.5.2.3 L-dihydroorotate + H2O
-
 Toxoplasma gondii N-carbamoyl-L-aspartate
-
 r
3.5.2.3 N-carbamoyl-L-aspartate
-
 Toxoplasma gondii L-dihydroorotate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.2.3 monomer 1 * 44200, SDS-PAGE Toxoplasma gondii
3.5.2.3 monomer 1 * 45600, gel filtration and laser light scattering Toxoplasma gondii

Synonyms

EC Number Synonyms Comment Organism
3.5.2.3 DHOase
-
 Saccharomyces cerevisiae
3.5.2.3 DHOase
-
 Mycobacterium leprae
3.5.2.3 DHOase
-
 Homo sapiens
3.5.2.3 DHOase
-
 Mesocricetus auratus
3.5.2.3 DHOase
-
 Toxoplasma gondii
3.5.2.3 DHOase
-
 Plasmodium berghei
3.5.2.3 DHOase
-
 Ustilago maydis
3.5.2.3 DHOase
-
 Arabidopsis thaliana
3.5.2.3 DHOase
-
 Escherichia coli
3.5.2.3 DHOase
-
 Trypanosoma cruzi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.3 5 9 in the biosynthetic direction Toxoplasma gondii
3.5.2.3 5 7.8 in the degradative direction Toxoplasma gondii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.3 Toxoplasma gondii isoelectric focusing
-
 5.72