Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zverlov, V.V.; Schantz, N.; Schmitt-Kopplin, P.; Schwarz, W.H.
    Two new major subunits in the cellulosome of Clostridium thermocellum: xyloglucanase Xgh74A and endoxylanase Xyn10D (2005), Microbiology, 151, 3395-3401.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.155 cellulosome
-
 Acetivibrio thermocellus 43263
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.155 81800
-
-
 Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.155 xyloglucan + H2O Acetivibrio thermocellus Xgh74 contributes to the in vivo degradation of the hemicellulose xyloglucan and xylan by the cellulosome of Clostridium thermocellum ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.155 Acetivibrio thermocellus
-
-
-
3.2.1.155 Acetivibrio thermocellus Q70DK5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.155 recombinant Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.155 beta-glucan + H2O 8% of the activity with xyloglucan Acetivibrio thermocellus beta-glucan oligosaccharides
-
 ?
3.2.1.155 carboxymethyl cellulose + H2O 5% of the activity with xyloglucan Acetivibrio thermocellus carboxymethyl cellulose oligosaccharides
-
 ?
3.2.1.155 additional information low activity on carboxymethyl cellulose and on glucuronoxylan. Lack of activity on amorphous cellulose Acetivibrio thermocellus ?
-
 ?
3.2.1.155 xyloglucan + H2O Xgh74 contributes to the in vivo degradation of the hemicellulose xyloglucan and xylan by the cellulosome of Clostridium thermocellum Acetivibrio thermocellus ?
-
 ?
3.2.1.155 xyloglucan + H2O the enzyme is a true endo-glucanase when it acts on linear substrates without bulky substituents in the polymer backbone. When the enzyme acts on polymeric substrate with bulky side chains, its mode of action is switched to exo-like Acetivibrio thermocellus xyloglucan oligosaccharides XXXG, XXLG and XLLG oligosaccharides ?
3.2.1.155 xyloglucan + H2O tamarind xyloglucan. Xgh74A hydrolyzes every fourth beta-1,4-glucan bond in the xyloglucan backbone, thus producing decorated cellotetraose units Acetivibrio thermocellus cellotetraose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.155 xyloglucanase Xgh74A
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.155 75
-
-
 Acetivibrio thermocellus
3.2.1.155 75
-
 hydrolysis of tamarind seed xyloglucan Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.155 75
-
 stable up to Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.155 6.4
-
 hydrolysis of tamarid xyloglucan Acetivibrio thermocellus
3.2.1.155 6.4
-
 hydrolysis of tamarind seed xyloglucan Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.155 5.7 7.8 50% of maximal activity at pH 5.7 and at pH 7.8 Acetivibrio thermocellus
3.2.1.155 5.7 7.8 more than 50% of maximal activity in the pH-range 5.7-7.8, hydrolysis of tamarind seed xyloglucan Acetivibrio thermocellus