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Literature summary extracted from

  • Zeitler, C.E.; Estes, M.K.; Venkataram Prasad, B.V.
    X-ray crystallographic structure of the Norwalk virus protease at 1.5-A resolution (2006), J. Virol., 80, 5050-5058.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.66 Glu 54 active role in proteolysis Norwalk virus

Application

EC Number Application Comment Organism
3.4.22.66 medicine Norwalk virus is the major cause of acute, epidemic, viral gastroenteritis Norwalk virus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.66 cloned into pET41 Ek/LIC and pET46 Ek/LIC and expressed in Escherichia coli BL21 (DE3) Norwalk virus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.66 by hanging drop vapor diffusion or sitting drop vapor diffusion at room temperature, to 1.5 and 2.2 A resolution Norwalk virus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.66 E54A Glu at the active centre Norwalk virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.66 4-(2-aminoethyl)-benzene-sulfonyl fluoride irreversible inhibition, soluble stable nontoxic alternative to phenylmethylsulfonyl fluoride Norwalk virus
3.4.22.66 phenylmethylsulfonyl fluoride
-
 Norwalk virus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.66 Norwalk virus Q83883
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.66 by nickel affinity chromatography or size exclusion chromatography Norwalk virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.66 ORF1 polyprotein + H2O
-
 Norwalk virus six nonstructural proteins for replication
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.66 dimer size exclusion chromatography results Norwalk virus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.66 NV protease
-
 Norwalk virus
3.4.22.66 NVPro
-
 Norwalk virus
3.4.22.66 viral cysteine protease
-
 Norwalk virus