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Literature summary extracted from
- New effective sources of the Staphylococcus simulans lysostaphin (2005), J. Biotechnol., 117, 203-213.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.75 | expressed in Escherichia coli | Staphylococcus simulans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.75 | Co2+ | 10 mM, 30% decrease of activity of enzyme produced with pET vector system and 20% decrease of activity of enzyme produced with pBAD vector system | Staphylococcus simulans |  |
| 3.4.24.75 | Cu2+ | 2 mM, 70-80% decrease of activity | Staphylococcus simulans | |
| 3.4.24.75 | additional information | no inhibitory effect of EDTA and 2 mM Co2+ | Staphylococcus simulans | |
| 3.4.24.75 | Ni2+ | 2 mM, 60% decrease of activity of enzyme produced with pET vector system and 20% decrease of activity of enzyme produced with pBAD vector system | Staphylococcus simulans | |
| 3.4.24.75 | Zn2+ | 2 mM, 70-80% decrease of activity | Staphylococcus simulans | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.75 | Staphylococcus simulans | P10547 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.75 | cobalt Sepharose column chromatography | Staphylococcus simulans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.75 | additional information | - |
970 U/mg | Staphylococcus simulans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.75 | Peptidoglycan + H2O | - |
Staphylococcus simulans | Fragments of peptidoglycan | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.75 | 45 | - |
- |
Staphylococcus simulans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.75 | 7.5 | - |
enzymeproduced with pBAD vector system | Staphylococcus simulans |
| 3.4.24.75 | 8 | - |
enzyme obtained from pET vector system | Staphylococcus simulans |
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Release 2023.1 (January 2023)
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