Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Takanashi, M.; Saito, T.
    Characterization of two 3-hydroxybutyrate dehydrogenases in poly(3-hydroxybutyrate)-degradable bacterium, Ralstonia pickettii T1 (2006), J. Biosci. Bioeng., 101, 501-507.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.30 expression in Escherichia coli Ralstonia pickettii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.30 0.1
-
 NAD+ 30°C Ralstonia pickettii
1.1.1.30 0.24
-
 NADH 30°C Ralstonia pickettii
1.1.1.30 0.31
-
 NAD+ 30°C Ralstonia pickettii
1.1.1.30 0.32
-
 NADH 30°C Ralstonia pickettii
1.1.1.30 0.34
-
 (R)-3-hydroxybutanoate 30°C Ralstonia pickettii
1.1.1.30 0.49
-
 (R)-3-hydroxybutanoate 30°C Ralstonia pickettii
1.1.1.30 1.1
-
 acetoacetate 30°C Ralstonia pickettii
1.1.1.30 1.3
-
 acetoacetate 30°C Ralstonia pickettii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.30 31000
-
 4 * 31000, SDS-PAGE Ralstonia pickettii
1.1.1.30 110000
-
 gel filtration Ralstonia pickettii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Ralstonia pickettii Q2PEN2
-
-
1.1.1.30 Ralstonia pickettii Q2PEN3
-
-
1.1.1.30 Ralstonia pickettii T1 Q2PEN2
-
-
1.1.1.30 Ralstonia pickettii T1 Q2PEN3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30
-
 Ralstonia pickettii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 85
-
-
 Ralstonia pickettii
1.1.1.30 180
-
-
 Ralstonia pickettii
1.1.1.30 360
-
 recombinant enzyme Ralstonia pickettii
1.1.1.30 450
-
 recombinant enzyme Ralstonia pickettii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Ralstonia pickettii acetoacetate + NADH + H+
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Ralstonia pickettii T1 acetoacetate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.30 tetramer 4 * 31000, SDS-PAGE Ralstonia pickettii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.30 BDH1
-
 Ralstonia pickettii
1.1.1.30 BDH2
-
 Ralstonia pickettii
1.1.1.30 D-(-)-3-hydroxybutyrate dehydrogenase
-
 Ralstonia pickettii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.30 58
-
 NADH 30°C Ralstonia pickettii
1.1.1.30 87
-
 NAD+ 30°C Ralstonia pickettii
1.1.1.30 100
-
 acetoacetate 30°C Ralstonia pickettii
1.1.1.30 110
-
 acetoacetate 30°C Ralstonia pickettii
1.1.1.30 180
-
 (R)-3-hydroxybutanoate 30°C Ralstonia pickettii
1.1.1.30 280
-
 NADH 30°C Ralstonia pickettii
1.1.1.30 310
-
 (R)-3-hydroxybutanoate 30°C Ralstonia pickettii
1.1.1.30 340
-
 NAD+ 30°C Ralstonia pickettii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.30 5
-
 reduction Ralstonia pickettii
1.1.1.30 6.5
-
 reduction Ralstonia pickettii
1.1.1.30 7.5
-
 oxidation Ralstonia pickettii
1.1.1.30 9
-
 oxidation Ralstonia pickettii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Ralstonia pickettii
1.1.1.30 NADH
-
 Ralstonia pickettii