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Literature summary extracted from

  • Masuo, N.; Yoshimune, K.; Ito, K.; Matsushima, K.; Koyama, Y.; Moriguchi, M.
    Micrococcus luteus K-3-type glutaminase from Aspergillus oryzae RIB40 is salt-tolerant (2005), J. Biosci. Bioeng., 100, 576-578.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.2 nutrition the enzyme from Aspergillus oryzae is important in soy sauce fermentation Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 NaCl 40% inhibition of the enzyme from strain RIB40 and 90% of the enzyme from strain MA-27-IM at 2.9 M, reduces the temperature stability of the enzyme Aspergillus oryzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.2 extracellular enzyme from strain MA-27-IM Aspergillus oryzae
-
-
3.5.1.2 intracellular enzyme from strain MA-27-IM Aspergillus oryzae 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.2 additional information the enzyme from strain RIB40 is salt-tolerant, while the enzyme from strain MA-27-IM is salt-sensitive Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.2 L-glutamine + H2O Aspergillus oryzae
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Aspergillus oryzae
-
 strains RIB40 and MA-27-IM
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 L-glutamine + H2O
-
 Aspergillus oryzae L-glutamate + NH3
-
 ?
3.5.1.2 additional information no activity with glutamine and hydroxylamine as substrates, no formation of L-glutamate-gamma-monohydroxamate Aspergillus oryzae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.2 AoGls
-
 Aspergillus oryzae
3.5.1.2 Micrococcus luteus K-3-type glutaminase only from strain RIB40, not from strain MA-27-IM Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 30
-
 enzyme from strain RIB40 Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.2 20 60
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.2 35
-
 30 min, pH 7.5, the enzyme from strain RIB40 is stable Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 8 9 enzyme from strain RIB40 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.2 5.5 11
-
 Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.2 7.5 8 30 min, 40°C, enzyme from strain RIB40, most stbale at Aspergillus oryzae