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Literature summary extracted from

  • Li, L.; Dworkowski, F.S.; Cook, P.F.
    Importance in catalysis of the 6-phosphate-binding site of 6-phosphogluconate in sheep liver 6-phosphogluconate dehydrogenase (2006), J. Biol. Chem., 281, 25568-25576.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.44 expressed in Escherichia coli strain M15 Ovis aries

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.44 K260A reduced activity compared to the wild type enzyme Ovis aries
1.1.1.44 R287A reduced activity compared to the wild type enzyme Ovis aries
1.1.1.44 R446A reduced activity compared to the wild type enzyme Ovis aries
1.1.1.44 T262A activity similar to the wild type enzyme Ovis aries
1.1.1.44 Y191A reduced activity compared to the wild type enzyme Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.0042
-
 NADP+ mutant enzyme T262A Ovis aries
1.1.1.44 0.005
-
 NADP+ wild type enzyme Ovis aries
1.1.1.44 0.014
-
 NADP+ mutant enzyme Y191A Ovis aries
1.1.1.44 0.016
-
 NADP+ mutant enzyme K260A Ovis aries
1.1.1.44 0.024
-
 6-phospho-D-gluconate mutant enzyme T262A Ovis aries
1.1.1.44 0.028
-
 6-phospho-D-gluconate wild type enzyme Ovis aries
1.1.1.44 0.064
-
 NADP+ mutant enzyme R446A Ovis aries
1.1.1.44 0.073
-
 NADP+ mutant enzyme R287A Ovis aries
1.1.1.44 0.15
-
 6-phospho-D-gluconate mutant enzyme K260A Ovis aries
1.1.1.44 1.04
-
 6-phospho-D-gluconate mutant enzyme Y191A Ovis aries
1.1.1.44 2 3 6-phospho-D-gluconate mutant enzyme R287A Ovis aries
1.1.1.44 3.4
-
 6-phospho-D-gluconate mutant enzyme R446A Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Ovis aries P00349
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44 Ni-NTA resin chromatography and ADP-agarose affinity column chromatography Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 liver
-
 Ovis aries
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.44 4°C, 50 mM triethanolamine and 2 mM beta-mercaptoethanol at pH 7.5, 1 year, no loss of activity Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6-phosphogluconate dehydrogenase
-
 Ovis aries
1.1.1.44 6PGDH
-
 Ovis aries

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.44 5.5 9.5 mutant Y191A measured by varying 6PG saturation level of NADP+ Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.44 NADP+
-
 Ovis aries