BRENDA - Enzyme Database

Molecular mechanism of the reduction of cysteine sulfinic acid of peroxiredoxin to cysteine by mammalian sulfiredoxin

Jeong, W.; Park, S.J.; Chang, T.S.; Lee, D.Y.; Rhee, S.G.; J. Biol. Chem. 281, 14400-14407 (2006)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.98.2
ATP
-
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
C99A
Cys99 replaced by Ala
Homo sapiens
1.8.98.2
C99S
Cys99 replaced by Ser
Homo sapiens
1.8.98.2
D57N
Asp57 replaced by Asn
Homo sapiens
1.8.98.2
D79N
Asp60 replaced by Asn
Homo sapiens
1.8.98.2
H99N
His99 replaced by Asn
Homo sapiens
1.8.98.2
K60R
Lys60 replaced by Arg
Homo sapiens
1.8.98.2
R100M
Arg100 replaced by Met
Homo sapiens
1.8.98.2
R50M
Arg50 replaced by Met
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.98.2
Mg2+
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.98.2
12000
-
SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.98.2
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
-
669451
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.98.2
0.003
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
at 30°C
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.98.2
ATP
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.8.98.2
C99A
Cys99 replaced by Ala
Homo sapiens
1.8.98.2
C99S
Cys99 replaced by Ser
Homo sapiens
1.8.98.2
D57N
Asp57 replaced by Asn
Homo sapiens
1.8.98.2
D79N
Asp60 replaced by Asn
Homo sapiens
1.8.98.2
H99N
His99 replaced by Asn
Homo sapiens
1.8.98.2
K60R
Lys60 replaced by Arg
Homo sapiens
1.8.98.2
R100M
Arg100 replaced by Met
Homo sapiens
1.8.98.2
R50M
Arg50 replaced by Met
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.98.2
Mg2+
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.98.2
12000
-
SDS-PAGE
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
-
669451
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.98.2
0.003
-
peroxiredoxin-(S-hydroxy-S-oxocysteine)
at 30°C
Homo sapiens