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Literature summary extracted from
- Plant gamma-glutamyl hydrolases and folate polyglutamates: characterization, compartmentation, and co-occurrence in vacuoles (2005), J. Biol. Chem., 280, 28877-28884.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.19.9 | AtGGH1 is expressed in truncated form in Escherichia coli | Arabidopsis thaliana |
| 3.4.19.9 | AtGGH2 is expressed in truncated form in Escherichia coli | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.9 | 0.00052 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana |  |
| 3.4.19.9 | 0.00079 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana | |
| 3.4.19.9 | 0.0015 | - |
p-aminobenzoylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana | |
| 3.4.19.9 | 0.00186 | - |
p-aminobenzoylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.19.9 | vacuole | - |
Beta vulgaris | 5773 | - |
| 3.4.19.9 | vacuole | - |
Pisum sativum | 5773 | - |
| 3.4.19.9 | vacuole | - |
Arabidopsis thaliana | 5773 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.9 | Arabidopsis thaliana | O65355 | - |
- |
| 3.4.19.9 | Beta vulgaris | - |
- |
- |
| 3.4.19.9 | Pisum sativum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.9 | recombinant AtGGH1 | Arabidopsis thaliana |
| 3.4.19.9 | recombinant AtGGH2 | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.19.9 | leaf | - |
Pisum sativum | - |
| 3.4.19.9 | root | - |
Beta vulgaris | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.9 | p-aminobenzoyl-penta-gamma-glutamate + H2O | AtGGH1 cleaves pentaglutamates mainly to di- and triglutamates | Arabidopsis thaliana | ? | - |
? | |
| 3.4.19.9 | p-aminobenzoyl-penta-gamma-glutamate + H2O | AtGGH2 cleaves pentaglutamates mainly to monoglutamates | Arabidopsis thaliana | ? | - |
? | |
| 3.4.19.9 | pteroyl-penta-gamma-glutamate + H2O | AtGGH1 cleaves pentaglutamates mainly to di- and triglutamates | Arabidopsis thaliana | ? | - |
? | |
| 3.4.19.9 | pteroyl-penta-gamma-glutamate + H2O | AtGGH2 cleaves pentaglutamates mainly monoglutamates | Arabidopsis thaliana | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.19.9 | dimer | AtGGH1 | Arabidopsis thaliana |
| 3.4.19.9 | dimer | AtGGH2 | Arabidopsis thaliana |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.19.9 | AtGGH1 | - |
Arabidopsis thaliana |
| 3.4.19.9 | AtGGH2 | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.9 | 0.04 | 1.97 | pteroylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana | |
| 3.4.19.9 | 1.1 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana | |
| 3.4.19.9 | 5.51 | - |
p-aminobenzoylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana | |
| 3.4.19.9 | 8.29 | - |
p-aminobenzoylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana | |
| 3.4.19.9 | 8.74 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH2 | Arabidopsis thaliana | |
| 3.4.19.9 | 19.46 | - |
pteroylpentaglutamate | 37°C, pH 6.0, AtGGH1 | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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