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Literature summary extracted from

  • Wu, Y.I.; Munshi, H.G.; Sen, R.; Snipas, S.J.; Salvesen, G.S.; Fridman, R.; Stack, M.S.
    Glycosylation broadens the substrate profile of membrane type 1 matrix metalloproteinase (2004), J. Biol. Chem., 279, 8278-8289.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.24.80 additional information glycolysation of MT1-MMP may protect against autolysis and thus stabilize ative MT1-MMP Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.80 expressed in COS-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.80 E240A loss of MT1-MMP activity Homo sapiens
3.4.24.80 additional information deletions delta Cat or point mutations CHO-1 and CHO-4, which preserve a relative electrophoretic mobility shift following desialylation similar to the wild type protein, moreover mutant CHO-4 is insensitive to sialidase A treatment, CHO-3 and CHO-4 mtuants are unable to effectively catalyze pro-MMP-2 activation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.80 GM6001 blocks conversion of active 50 kDa wild type MT1-MMP to a 37 kDa species, inhibits autolysis of both wild type and CHO-4 MT1-MMP in a dose-dependent manner and with a similar inhibitory profile Homo sapiens
3.4.24.80 TIMP-2 under in vivo conditions primary inhibitor of MT1-MMP, increases endocytosis of wild type MT1-MMP Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.80 50000
-
 active MT1-MMP species, SDS-PAGE Homo sapiens
3.4.24.80 64000
-
 purified protein, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.80 Homo sapiens P50281
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.80 affinity purification from COS-7 cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.80 MDA-MB-231 cell
-
 Homo sapiens
-
3.4.24.80 additional information ovarian cancer cell lines DOV13, OVCA429, OVCA433 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.80 pro-MMP-2 + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.80 Type I collagen + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.80 membrane type 1-matrix metalloproteinase
-
 Homo sapiens
3.4.24.80 MT1-MMP
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.80 0.001 0.01 GM6001 partial blockade of autolysis of both wild type and CHO-4 mutant MT1-MMP Homo sapiens
3.4.24.80 0.1
-
 GM6001 complete blockade of autolysis of both wild type and CHO-4 mutant MT1-MMP Homo sapiens