EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.64 | - |
Pisum sativum |
2.7.7.64 | His-tagged thioredoxin fusion protein expressed in Escherichia coli BL21gold strain | Pisum sativum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.64 | 0.048 | - |
UTP | pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 0.048 | - |
UTP | D-glucose 1-phosphate, pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 0.25 | - |
diphosphate | pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 0.34 | - |
UDP-glucose | pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 0.34 | - |
D-glucose 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 0.34 | - |
UDP-D-glucose | pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 0.48 | - |
D-glucuronic acid 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 0.58 | - |
D-galactose 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 0.96 | - |
L-arabinose 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 0.96 | - |
arabinose 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
2.7.7.64 | 1.98 | - |
D-xylose 1-phosphate | pH 7.0, 35°C | Pisum sativum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.64 | Mg2+ | - |
Pisum sativum | |
2.7.7.64 | Mg2+ | absolute requirement for divalent cation, maximal activity in presence of Mg2+ or Mn2+. Maximal activity in presence of 2 mM Mg2+ | Pisum sativum | |
2.7.7.64 | Mn2+ | 75% activity in comarison with Mg2+ | Pisum sativum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.64 | 66040 | - |
x * 66040, calculated from sequence | Pisum sativum |
2.7.7.64 | 67000 | - |
x * 67000, SDS-PAGE | Pisum sativum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.64 | UTP + a monosaccharide 1-phosphate | Pisum sativum | the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways | diphosphate + UDP-monosaccharide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.64 | Pisum sativum | Q5W915 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.64 | ammonium sulfate fractionation, ion exchange chromatography (DEAE), hydrophobic interaction chromatography, gel filtration and ion exchange chromatography (DEAE), recombinant protein: immobilized metal ion affinity chromatography, splitting off the fused thioredoxin and His-tag, ion exchange chromatography (DEAE) | Pisum sativum |
2.7.7.64 | recombinant | Pisum sativum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.64 | sprout | - |
Pisum sativum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.64 | 30 | - |
UDP-L-arabinose pyrophosphorylase, pH 7.0, 35°C | Pisum sativum |
2.7.7.64 | 63.9 | - |
- |
Pisum sativum |
2.7.7.64 | 63.9 | - |
UDP-D-glucose pyrophosphorylase, pH 7.0, 35°C | Pisum sativum |
2.7.7.64 | 95 | - |
recombinant UDP-glucose pyrophosphorylase, pH 7.0, 35°C | Pisum sativum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.64 | diphosphate + UDP-D-glucose | - |
Pisum sativum | UTP + D-glucose 1-phosphate | - |
r | |
2.7.7.64 | UTP + a monosaccharide 1-phosphate | the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways | Pisum sativum | diphosphate + UDP-monosaccharide | - |
? | |
2.7.7.64 | UTP + arabinose 1-phosphate | 70.5% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-arabinose | - |
r | |
2.7.7.64 | UTP + D-galactose 1-phosphate | - |
Pisum sativum | diphosphate + UDP-galactose | - |
? | |
2.7.7.64 | UTP + D-galactose 1-phosphate | 116.2% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-galactose | - |
r | |
2.7.7.64 | UTP + D-glucose 1-phosphate | - |
Pisum sativum | diphosphate + UDP-glucose | - |
r | |
2.7.7.64 | UTP + D-glucose 1-phosphate | UTP specific | Pisum sativum | diphosphate + UDP-glucose | - |
r | |
2.7.7.64 | UTP + D-glucuronic acid 1-phosphate | 71.3% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-D-glucuronic acid | - |
r | |
2.7.7.64 | UTP + D-glucuronic acid 1-phosphate | - |
Pisum sativum | diphosphate + UDP-glucuronic acid | - |
? | |
2.7.7.64 | UTP + D-xylose 1-phosphate | - |
Pisum sativum | diphosphate + UDP-alpha-D-xylopyranose | - |
? | |
2.7.7.64 | UTP + D-xylose 1-phosphate | 35.9% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-xylose | - |
r | |
2.7.7.64 | UTP + L-arabinose 1-phosphate | - |
Pisum sativum | diphosphate + UDP-beta-L-arabinopyranose | - |
? | |
2.7.7.64 | UTP + N-acetyl-D-glucosamine 1-phosphate | 4.3% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-N-acetylglucosamine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.64 | ? | x * 67000, SDS-PAGE | Pisum sativum |
2.7.7.64 | ? | x * 66040, calculated from sequence | Pisum sativum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.64 | PsUSP | - |
Pisum sativum |
2.7.7.64 | UDP-sugar pyrophosphorylase | - |
Pisum sativum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.64 | 45 | - |
- |
Pisum sativum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.64 | additional information | - |
loss of activity of more than 99% at 55°C | Pisum sativum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.64 | 6.5 | 7.5 | - |
Pisum sativum |
2.7.7.64 | 7 | - |
pH 6.5-7.5, formation of UDP-glucose | Pisum sativum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.64 | 6.5 | 8 | 80% loss of activity below pH 5.0 | Pisum sativum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.7.64 | Pisum sativum | calculated | - |
5.82 |