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Literature summary extracted from

  • Yoshimura, Y.; Tani, M.; Okino, N.; Iida, H.; Ito, M.
    Molecular cloning and functional analysis of zebrafish neutral ceramidase (2004), J. Biol. Chem., 279, 44012-44022.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 DNA and amino acid sequence determination and analysis, overexpression of C-terminally myc-tagged enzyme in CHOP cells and zebrafish BRF41 cells in endoplasmic reticulum and Golgi apparatus, as well as in plasma membranes Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.23 additional information enzyme knockout, via antisense construct using three different antisense morpholino oligonucleotides (AMOs 1, 2, and 3) that are designed based on sequences at different sites of the 5'-untranslated region, leads to an increase in the number of zebrafish embryos with severe morphological qand cellular abnormalities such as abnormal morphogenesis inhead and tail, pericardiac edema, defect of blood cell circulation, and an increase in apoptotic cells, phenotype, overview Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 microvillus of absorptive epithelial cells of the intestine Danio rerio 5902
-
3.5.1.23 plasma membrane type II integral protein with its N-terminal end anchored to the plasma membrane and C-terminal end exposed to the extracellular space Danio rerio 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 82500
-
 x * 110000, glycosylated recombinant enzyme, SDS-PAGE, x * 82500, deglycosylated recombinant enzyme, SDS-PAGE Danio rerio
3.5.1.23 110000
-
 x * 110000, glycosylated recombinant enzyme, SDS-PAGE, x * 82500, deglycosylated recombinant enzyme, SDS-PAGE Danio rerio

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Danio rerio
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.23 glycoprotein eight potential N-glycosylation sites, four potential O-glycosylation sites Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 brain
-
 Danio rerio
-
3.5.1.23 fin
-
 Danio rerio
-
3.5.1.23 muscle
-
 Danio rerio
-
3.5.1.23 small intestine
-
 Danio rerio
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 additional information
-
-
 Danio rerio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
-
 Danio rerio 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 110000, glycosylated recombinant enzyme, SDS-PAGE, x * 82500, deglycosylated recombinant enzyme, SDS-PAGE Danio rerio

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 neutral ceramidase
-
 Danio rerio
3.5.1.23 znCD
-
 Danio rerio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
 assay at Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 7.5
-
 assay at Danio rerio