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Literature summary extracted from

  • Cao, J.; Kozarekar, P.; Pavlaki, M.; Chiarelli, C.; Bahou, W.F.; Zucker, S.
    Distinct roles for the catalytic and hemopexin domains of membrane type 1-matrix metalloproteinase in substrate degradation and cell migration (2004), J. Biol. Chem., 279, 14129-14139.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.24.80 medicine MT1-MMP initiates substrate degradation and enhances cell migration, hemopexin-like domain and a nonenzymatic component of the catalytic domain of MT1-MMP are essential for MT1-MMP-mediated cell migration, Rac1 participates in the MT1-MMP signal transduction pathway Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.80 cloned into pSG5 vector, expression in COS-1 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.80 E240A expressed on transfected cell surface but fails to activate pMMP-2 Homo sapiens
3.4.24.80 K44A dominant negative dynamin mutation controlled by a separate cytomegalovirus promoter MT1/K44A, leading to increased substrate digestion that is contributed by enhanced cell migration resulting from the accumulation of MT1-MMP ant the plasma membrane Homo sapiens
3.4.24.80 additional information mutant Sol.MT, fails to activate pMMP-2, mutant MTdeltaC, pMMP-2 activation as compared with wild type, neither cell type-dependent nor extracellular matrix component-dependent Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.80 additional information cells cotransfected with TIMP-1 cDNA along with MT1-MMP and pMMP-2 cDNAs result in partial inhibition of substrate degradation, down to the basal digestion level resulting from MT1-MMP alone, wortmannin and PD98059 do not interfer with MT1-MMP-induced cell migration, CDC42 (N17) and RhoA(N19) have no effect on MT1-MMP-dependent cell migration Homo sapiens
3.4.24.80 Rac1(N17Rac) coexpression wit MT1-MMP cDNAs leads to complete inhibition of migration Homo sapiens
3.4.24.80 TIMP-2 totally abolishes substrate degradation Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.80 cytoplasm short cytoplasmic tail Homo sapiens 5737
-
3.4.24.80 perinuclear space highest amounts Homo sapiens
-
-
3.4.24.80 plasma membrane plasma membrane anchor Homo sapiens 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.80 90000
-
 MT1-GFP fusion protein, Western blot analysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.80 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.80 breast cancer cell line
-
 Homo sapiens
-
3.4.24.80 LNCaP cell
-
 Homo sapiens
-
3.4.24.80 MDA-MB-231 cell
-
 Homo sapiens
-
3.4.24.80 prostate cancer cell line
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.80 Fibrin + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.80 Fibrinogen + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.80 Fibronectin + H2O cell surface active pMMP-2 cDNAs bound to MT1-MMP enhances substrate digestion, cytoplasmic tail of MT1-MMP not required for digestion Homo sapiens ?
-
 ?
3.4.24.80 Gelatin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.80 membrane type 1 matrix metalloproteinase
-
 Homo sapiens
3.4.24.80 MT1-MMP
-
 Homo sapiens