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Literature summary extracted from

  • Zillman, M.; Gorovsky, M.A.; Phizicky, E.M.
    HeLa cells contain a 2'-phosphate-specific phosphotransferase similar to a yeast enzyme implicated in tRNA splicing (1992), J. Biol. Chem., 267, 10289-10294.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.160 Homo sapiens
-
-
-
2.7.1.160 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.160 partially purified by chromatography on heparin-agarose Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.160 HeLa cell
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.160 -20, Tris-HCl buffer, pH. 8.0, 50% glycerol, several months Homo sapiens
2.7.1.160 -80°C, Tris-HCl buffer, pH 8.0, 20% glycerol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.160 2'-phospho-[ligated tRNA] + NAD+ minimal substrate is a dinucleotide containing an internal 2' phosphate, 600fold more specific for 2'phosphates than for eihter 5'- or 3'-terminal phosphates, 20fold more specific for internal 2' phosphates than for terminal 2' 3', or 2'3'-cyclic phosphates, at least 50fold more specific for internal 2' phosphates than terminal 2' phosphates Homo sapiens mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?
2.7.1.160 2'-phospho-[ligated tRNA] + NAD+ minimal substrate is a dinucleotide containing an internal 2'-phosphate Saccharomyces cerevisiae mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.160 HeLa cell 2'-phosphotransferase
-
 Homo sapiens
2.7.1.160 yeast 2'-phosphotransferase
-
 Saccharomyces cerevisiae