Literature summary extracted from

Zhang, X.; Zhang, S.; Hao, F.; Lai, X.; Yu, H.; Huang, Y.; Wang, H.
Expression, purification and properties of shikimate dehydrogenase from Mycobacterium tuberculosis (2005), J. Biochem. Mol. Biol., 38, 624-631.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.1.1.25	Nonidet P-40	120-190% activity of AroE at 0.1-0.5%	Mycobacterium tuberculosis
1.1.1.25	Triton X-100	120-190% activity at 0.1-0.5%	Mycobacterium tuberculosis

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.25	gene aroE, expression of His-tagged AroE in Escherichia coli strain BL21(DE3)	Mycobacterium tuberculosis

General Stability

EC Number	General Stability	Organism
1.1.1.25	the purified recombinant enzyme is stable to freezing	Mycobacterium tuberculosis

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.25	SDS	nearly complete inactivation of AroE at 0.02%	Mycobacterium tuberculosis

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.25	additional information	-	additional information	kinetic profile of the recombinant AroE, overview	Mycobacterium tuberculosis
1.1.1.25	0.0295	-	NADP+	pH 9.0, 25°C	Mycobacterium tuberculosis
1.1.1.25	0.063	-	shikimate	pH 9.0, 25°C	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.25	shikimate + NADP+	Mycobacterium tuberculosis	-	3-dehydroshikimate + NADPH + H+	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.25	Mycobacterium tuberculosis	-	gene aroE	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.25	recombinant His-tagged ARoE from Escherichia coli strain BL21(DE3) by nickel affinity chromatograohy	Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.25	826	-	purified recombinant AroE	Mycobacterium tuberculosis

Storage Stability

EC Number	Storage Stability	Organism
1.1.1.25	-70°C, purified recombinant enzyme, stable for several months	Mycobacterium tuberculosis
1.1.1.25	4°C, purified recombinant enzyme, stable for 2 weeks	Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.25	shikimate + NADP+	-	Mycobacterium tuberculosis	3-dehydroshikimate + NADPH + H+	-	r

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.25	More	secondary structure analysis of ARoE, percentages for alpha-helix, beta-sheet, beta-turn, and random coil are 29.2%, 9.3%, 32.7%, and 28.8%, respectively, contains the highly conserved motif G-X-(N/S)-V-(T/S)-X-PX-K	Mycobacterium tuberculosis

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.25	AroE	-	Mycobacterium tuberculosis
1.1.1.25	SD	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.1.25	63	-	-	Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.1.1.25	87	-	purified recombinant AroE, half-life: 2 h	Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.1.1.25	399	-	NADP+	pH 9.0, 25°C	Mycobacterium tuberculosis
1.1.1.25	399	-	shikimate	pH 9.0, 25°C	Mycobacterium tuberculosis

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.25	9	12	-	Mycobacterium tuberculosis

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.25	NADP+	-	Mycobacterium tuberculosis
1.1.1.25	NADPH	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)