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Literature summary extracted from

  • Maruyama, K.; Akita, K.; Naitou, C.; Yoshida, M.; Kitamura, T.
    Purification and characterization of an esterase hydrolyzing monoalkyl phthalates from Micrococcus sp. YGJ1 (2005), J. Biochem., 137, 27-32.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.1 in absence of 2-mercaptoethanol, purified enzymes are rapidly inactivated at 25°C Micrococcus sp.
3.1.1.1 unstable in crude extract, 10% v/v ethylene glycol and 10 mM 2-mercaptoethanol or 1 mM dithiothreitol stabilize Micrococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 CuSO4 1 mM, 23% residual activity, isoform E1, 10% residual activity, isoform E2 Micrococcus sp.
3.1.1.1 diethyldicarbonate 1 mM, 1.6% residual activity, isoform E1, 2.4% residual activity, isoform E2 Micrococcus sp.
3.1.1.1 ethanol inhibitory above 10% v/V Micrococcus sp.
3.1.1.1 HgCl2 complete inhibition Micrococcus sp.
3.1.1.1 additional information not inhibitory: EDTA, 2,2’-dipyridyl, 1,10-phenanthroline, 8-hydroxyquinoline, Tiron, at 1 mM each Micrococcus sp.
3.1.1.1 p-chloromercuribenzoate complete inhibition Micrococcus sp.
3.1.1.1 phenylmethylsulfonyl fluoride 1 mM, 79% residual activity, isoform E1, 70% residual activity, isoform E2 Micrococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 1.39
-
 monopentyl phthalate isoform E1, pH 7.5, 30°C Micrococcus sp.
3.1.1.1 1.67
-
 monobutyl phthalate isoform E1, pH 7.5, 30°C Micrococcus sp.
3.1.1.1 2.63
-
 monopentyl phthalate isoform E2, pH 7.5, 30°C Micrococcus sp.
3.1.1.1 2.7
-
 monobutyl phthalate isoform E2, pH 7.5, 30°C Micrococcus sp.
3.1.1.1 5.88
-
 monopropyl phthalate isoform E1, pH 7.5, 30°C Micrococcus sp.
3.1.1.1 6.25
-
 monopropyl phthalate isoform E2, pH 7.5, 30°C Micrococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 27000
-
 2 * 27000, SDS-PAGE, both E1 and E2 Micrococcus sp.
3.1.1.1 60000
-
 gel filtration, both E1 and E2 Micrococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Micrococcus sp.
-
 isoforms E1 and E2
-
3.1.1.1 Micrococcus sp. YGJ1
-
 isoforms E1 and E2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 butyl benzoate + H2O at 5-6% of the rate of monobutyl phthalate Micrococcus sp. benzoate + butanol
-
 ?
3.1.1.1 butyl benzoate + H2O at 5-6% of the rate of monobutyl phthalate Micrococcus sp. YGJ1 benzoate + butanol
-
 ?
3.1.1.1 monobutyl phthalate + H2O
-
 Micrococcus sp. phthalate + butanol
-
 ?
3.1.1.1 monobutyl phthalate + H2O
-
 Micrococcus sp. YGJ1 phthalate + butanol
-
 ?
3.1.1.1 monopentyl phthalate + H2O at 50% of the rate of monobutyl phthalate Micrococcus sp. phthalate + pentanol
-
 ?
3.1.1.1 monopentyl phthalate + H2O at 50% of the rate of monobutyl phthalate Micrococcus sp. YGJ1 phthalate + pentanol
-
 ?
3.1.1.1 monopropyl phthalate + H2O at 12-19% of the rate of monobutyl phthalate Micrococcus sp. phthalate + propanol
-
 ?
3.1.1.1 monopropyl phthalate + H2O at 12-19% of the rate of monobutyl phthalate Micrococcus sp. YGJ1 phthalate + propanol
-
 ?
3.1.1.1 additional information no substrate: 1-naphthyl acetate Micrococcus sp. ?
-
 ?
3.1.1.1 additional information no substrate: 1-naphthyl acetate Micrococcus sp. YGJ1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 dimer 2 * 27000, SDS-PAGE, both E1 and E2 Micrococcus sp.
3.1.1.1 More N-terminal amino acid sequence is identical for E1 and E2 Micrococcus sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 MAP esterase
-
 Micrococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 40
-
 both E1 and E2 Micrococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 27 55 50% of maximum activity within this range, both E1 and E2 Micrococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7.5
-
 both E1 and E2 Micrococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 6.5 7.8 50% of maximum activity within this range, both E1 and E2 Micrococcus sp.