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Literature summary extracted from

  • Umezawa, Y.; Yokoyama, K.; Kikuchi, Y.; Date, M.; Ito, K.; Yoshimoto, T.; Matsui, H.
    Novel prolyl tri/tetra-peptidyl aminopeptidase from Streptomyces mobaraensis: substrate specificity and enzyme gene cloning (2004), J. Biochem., 136, 293-300.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.12
-
 Streptomyces mobaraensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.12 1,10-phenanthroline 5 mM, 67% residual activity Streptomyces mobaraensis
3.4.14.12 Pefabloc SC 4 mM, 60% residual activity Streptomyces mobaraensis
3.4.14.12 Phenylmethylsulfonylfluoride 1 mM, no residual activity Streptomyces mobaraensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.12 0.072
-
 Gly-L-Ala-L-Pro-beta-naphthylamide pH 6.5, 30°C Streptomyces mobaraensis
3.4.14.12 0.26
-
 Gly-L-Ala-Gly-L-Pro-beta-naphthylamide pH 6.5, 30°C Streptomyces mobaraensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.12 cell wall connnected to wall through ionic interactions Streptomyces mobaraensis 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.12 50000
-
 x * 50000, SDS-PAGE Streptomyces mobaraensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.12 Streptomyces mobaraensis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.14.12 proteolytic modification sequence contains an N-terminal signal peptide of 33 amino acids followed by mature protein of 444 amino acids Streptomyces mobaraensis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.12
-
 Streptomyces mobaraensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.12 mycelium
-
 Streptomyces mobaraensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.12 Gly-L-Ala-Gly-L-Pro-beta-naphthylamide + H2O
-
 Streptomyces mobaraensis Gly-L-Ala-Gly-L-Pro + beta-naphthylamine
-
 ?
3.4.14.12 Gly-L-Ala-L-Pro-4-nitroanilide + H2O 32% of the activity with L-Ala-L-Ala-L-Pro-4-nitroanilide Streptomyces mobaraensis Gly-L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.14.12 Gly-L-Ala-L-Pro-beta-naphthylamide + H2O
-
 Streptomyces mobaraensis Gly-L-Ala-L-Pro + beta-naphthylamine
-
 ?
3.4.14.12 L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Streptomyces mobaraensis L-Ala-L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.14.12 L-Ala-L-Phe-L-Pro-4-nitroanilide + H2O 24% of the activity with L-Ala-L-Ala-L-Pro-4-nitroanilide Streptomyces mobaraensis L-Ala-L-Phe-L-Pro + 4-nitroaniline
-
 ?
3.4.14.12 L-Lys-L-Pro-L-Pro-4-nitroanilide + H2O 26% of the activity with L-Ala-L-Ala-L-Pro-4-nitroanilide Streptomyces mobaraensis L-Pro-L-Pro-L-Pro + 4-nitroaniline
-
 ?
3.4.14.12 additional information enzyme exhibits tripeptidyl peptidase and tetrapeptidyl peptidase activity, but no other exo- or endo-activites Streptomyces mobaraensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.12 ? x * 50000, SDS-PAGE Streptomyces mobaraensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.12 45
-
-
 Streptomyces mobaraensis
3.4.14.12 50
-
 significant decrease in activity above Streptomyces mobaraensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.12 30
-
 stable Streptomyces mobaraensis
3.4.14.12 40
-
 significant loss of stability above Streptomyces mobaraensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.12 43
-
 Gly-L-Ala-Gly-L-Pro-beta-naphthylamide pH 6.5, 30°C Streptomyces mobaraensis
3.4.14.12 115
-
 Gly-L-Ala-L-Pro-beta-naphthylamide pH 6.5, 30°C Streptomyces mobaraensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.12 6 6.5
-
 Streptomyces mobaraensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.12 4.3 8.3
-
 Streptomyces mobaraensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.14.12 Streptomyces mobaraensis isoelectric focusing
-
 10.2