Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Koreishi, M.; Zhang, D.; Imanaka, H.; Imamura, K.; Adachi, S.; Matsuno, R.; Nakanishi, K.
    A novel acylase from Streptomyces mobaraensis that efficiently catalyzes hydrolysis/synthesis of capsaicins as well as N-acyl-L-amino acids and N-acyl-peptides (2006), J. Agric. Food Chem., 54, 72-78.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.1.14 Co2+ activates and stabilizes Streptomyces mobaraensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.14 1,10-phenanthroline 24% inhibition Streptomyces mobaraensis
3.5.1.14 EDTA 13% inhibition Streptomyces mobaraensis
3.5.1.14 additional information no effects by GSH, L-Cys, (NH4)6Mo7O24, PMSF, PCMB, DTT, MgSO4, AgNO3, CaCl2, FeSO4, ZnCl2, and CuSO4 Streptomyces mobaraensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.14 Co2+ activates and stabilizes, 148% activity at 0.1 mM, tightly bound to the enzyme Streptomyces mobaraensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.14 19000
-
 1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer Streptomyces mobaraensis
3.5.1.14 61000
-
 1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer Streptomyces mobaraensis
3.5.1.14 75000
-
 gel filtration Streptomyces mobaraensis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Streptomyces mobaraensis
-
 i.e. Streptomyces luteoreticuli, strains NBRC/IFO 13819 and 13422
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14 native enzyme 1300fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, hydroxyapatite chromatography, and a second step of dialysis Streptomyces mobaraensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.14 22
-
 purified enzyme, substrate N-lauroyl-L-tryptophan Streptomyces mobaraensis
3.5.1.14 25
-
 purified enzyme, substrate N-lauroyl-L-phenylalanine Streptomyces mobaraensis
3.5.1.14 70
-
 purified enzyme, substrate N-lauroyl-L-glutaminyl-L-lysine Streptomyces mobaraensis
3.5.1.14 87
-
 purified enzyme, substrate N-lauroyl-L-valine Streptomyces mobaraensis
3.5.1.14 90
-
 purified enzyme, substrate N-lauroyl-L-glutamyl-glycine Streptomyces mobaraensis
3.5.1.14 90
-
 purified enzyme, substrate N-lauroyl-L-isoleucine Streptomyces mobaraensis
3.5.1.14 110
-
 purified enzyme, substrate N-lauroyl-L-glutaminyl-glycine Streptomyces mobaraensis
3.5.1.14 118
-
 purified enzyme, substrate N-lauroyl-L-histidine Streptomyces mobaraensis
3.5.1.14 129
-
 purified enzyme, substrate N-lauroyl-L-leucine Streptomyces mobaraensis
3.5.1.14 138
-
 purified enzyme, substrate N-lauroyl-L-aspartic acid Streptomyces mobaraensis
3.5.1.14 145
-
 purified enzyme, substrate N-lauroyl-L-tyrosine Streptomyces mobaraensis
3.5.1.14 177
-
 purified enzyme, substrate N-lauroyl-L-methionine Streptomyces mobaraensis
3.5.1.14 194
-
 purified enzyme, substrate N-lauroyl-L-arginine Streptomyces mobaraensis
3.5.1.14 196
-
 purified enzyme, substrate N-lauroyl-L-glutamic acid Streptomyces mobaraensis
3.5.1.14 210
-
 purified enzyme, substrate capsaicin Streptomyces mobaraensis
3.5.1.14 221
-
 purified enzyme, substrate N-lauroyl-L-glutamine Streptomyces mobaraensis
3.5.1.14 222
-
 purified enzyme, substrate N-lauroyl-glycine Streptomyces mobaraensis
3.5.1.14 227
-
 purified enzyme, substrate N-lauroyl-L-asparagine Streptomyces mobaraensis
3.5.1.14 230
-
 purified enzyme, substrate N-lauroyl-L-threonine Streptomyces mobaraensis
3.5.1.14 236
-
 purified enzyme, substrate N-lauroyl-L-alanine Streptomyces mobaraensis
3.5.1.14 244
-
 purified enzyme, substrate N-lauroyl-L-lysine Streptomyces mobaraensis
3.5.1.14 295
-
 purified enzyme, substrate N-lauroyl-L-serine Streptomyces mobaraensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 8-methyl-N-vanillyl-6-nonenamide + H2O i.e. capsaicin Streptomyces mobaraensis ?
-
 ?
3.5.1.14 additional information substrate synthesis in an aqueous/n-hexane biphasic system Streptomyces mobaraensis ?
-
 ?
3.5.1.14 N-lauroyl-glycine + H2O
-
 Streptomyces mobaraensis laurate + glycine
-
 ?
3.5.1.14 N-lauroyl-L-alanine + H2O
-
 Streptomyces mobaraensis laurate + L-alanine
-
 ?
3.5.1.14 N-lauroyl-L-arginine + H2O
-
 Streptomyces mobaraensis laurate + L-arginine
-
 ?
3.5.1.14 N-lauroyl-L-asparagine + H2O
-
 Streptomyces mobaraensis laurate + L-asparagine
-
 ?
3.5.1.14 N-lauroyl-L-aspartate + H2O
-
 Streptomyces mobaraensis laurate + L-aspartate
-
 ?
3.5.1.14 N-lauroyl-L-glutamate + H2O
-
 Streptomyces mobaraensis laurate + L-glutamate
-
 ?
3.5.1.14 N-lauroyl-L-glutamine + H2O
-
 Streptomyces mobaraensis laurate + L-glutamine
-
 ?
3.5.1.14 N-lauroyl-L-glutaminyl-glycine + H2O
-
 Streptomyces mobaraensis laurate + L-glutaminyl-glycine
-
 ?
3.5.1.14 N-lauroyl-L-glutaminyl-L-lysine + H2O
-
 Streptomyces mobaraensis laurate + L-glutaminyl-L-lysine
-
 ?
3.5.1.14 N-lauroyl-L-glutamyl-glycine + H2O
-
 Streptomyces mobaraensis laurate + L-glutamyl-glycine
-
 ?
3.5.1.14 N-lauroyl-L-histidine + H2O
-
 Streptomyces mobaraensis laurate + L-histidine
-
 ?
3.5.1.14 N-lauroyl-L-isoleucine + H2O
-
 Streptomyces mobaraensis laurate + L-isoleucine
-
 ?
3.5.1.14 N-lauroyl-L-leucine + H2O
-
 Streptomyces mobaraensis laurate + L-leucine
-
 ?
3.5.1.14 N-lauroyl-L-methionine + H2O
-
 Streptomyces mobaraensis laurate + L-methionine
-
 ?
3.5.1.14 N-lauroyl-L-phenylalanine + H2O
-
 Streptomyces mobaraensis laurate + L-phenylalanine
-
 ?
3.5.1.14 N-lauroyl-L-serine + H2O best substrate Streptomyces mobaraensis laurate + L-serine
-
 ?
3.5.1.14 N-lauroyl-L-threonine + H2O
-
 Streptomyces mobaraensis laurate + L-threonine
-
 ?
3.5.1.14 N-lauroyl-L-tryptophan + H2O
-
 Streptomyces mobaraensis laurate + L-tryptophan
-
 ?
3.5.1.14 N-lauroyl-L-tyrosine + H2O
-
 Streptomyces mobaraensis laurate + L-tyrosine
-
 ?
3.5.1.14 N-lauroyl-L-valine + H2O
-
 Streptomyces mobaraensis laurate + L-valine
-
 ?
3.5.1.14 Nalpha-lauroyl-L-lysine + H2O
-
 Streptomyces mobaraensis laurate + L-lysine
-
 ?
3.5.1.14 Nepsilon-lauroyl-L-lysine + H2O
-
 Streptomyces mobaraensis laurate + L-lysine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.14 dimer 1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer Streptomyces mobaraensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.14 55
-
-
 Streptomyces mobaraensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.14 55
-
 1 h, pH 7.8, stable, above 55°C the enzyme is stable only in presence of Co2+ Streptomyces mobaraensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.14 8
-
-
 Streptomyces mobaraensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.14 5 8 50 mm Tris, 0.5 mM Co2+, 37°C, stable Streptomyces mobaraensis