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Literature summary extracted from

  • Delpino, M.V.; Marchesini, M.I.; Estein, S.M.; Comerci, D.J.; Cassataro, J.; Fossati, C.A.; Baldi, P.C.
    A bile salt hydrolase of Brucella abortus contributes to the establishment of a successful infection through the oral route in mice (2007), Infect. Immun., 75, 299-305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.24 gene cgh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain BL21(DE3) Brucella abortus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.24 additional information construction of an isogenic deletion mutant strain DELTAcgh, the growth of the the cgh mutant is inhibited by addition of 5% or 10% bile acid, but is similar to the wild-type strain in absence of bile acids, the mutant enzyme does not confer resistance to bile acids to the organism Brucella abortus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.24 additional information Brucella abortus the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts ?
-
 ?
3.5.1.24 additional information Brucella abortus 2308 the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.24 Brucella abortus Q2YS20
-
-
3.5.1.24 Brucella abortus 2308 Q2YS20
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.24 recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, and polymyxin B resin chromatography, the latter to eliminate lipopolysaccharide contamination Brucella abortus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.24 glycocholate + H2O
-
 Brucella abortus glycine + cholate
-
 ?
3.5.1.24 glycocholate + H2O
-
 Brucella abortus 2308 glycine + cholate
-
 ?
3.5.1.24 additional information the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts Brucella abortus ?
-
 ?
3.5.1.24 additional information the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts Brucella abortus 2308 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.24 bile salt hydrolase
-
 Brucella abortus
3.5.1.24 CGH
-
 Brucella abortus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.24 37
-
 assay at Brucella abortus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.24 7 8
-
 Brucella abortus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.24 6 9 activity range Brucella abortus