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Literature summary extracted from
- Atrolysin E (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 674-676.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.24.44 | medicine | one of the most hemorrhagic metalloproteinases of the P-II class in the venom, causes extensive hemorrhage in muscle tissue and extensive degeneration of capillaries, degradation of basement membrane proteins and surrounding stroma, its free disintegrin domain possesses platelet-aggregation inhibitory activity | Crotalus atrox |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.44 | produced with a CMV expression vector in 293 cells | Crotalus atrox |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.44 | 1,10-phenanthroline | inhibits proteolytic and hemorrhagic activities | Crotalus atrox |  |
| 3.4.24.44 | alpha2-Macroglobulin | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
| 3.4.24.44 | antihemorrhagins from serum of Crotalus atrox | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
| 3.4.24.44 | EDTA | inhibits proteolytic and hemorrhagic activities | Crotalus atrox | |
| 3.4.24.44 | synthetic carboxyalkyl peptide | effective inhibitor | Crotalus atrox | |
| 3.4.24.44 | venom-derived pyroglutamyl-containing peptide | effective inhibitor | Crotalus atrox |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.44 | Ca2+ | low concentrations stabilize proteolytic activity | Crotalus atrox | |
| 3.4.24.44 | Zn2+ | can be replaced by Co2+, which does not significantly alter the proteolytic activity | Crotalus atrox | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.44 | 25700 | - |
SDS-PAGE | Crotalus atrox |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.44 | Crotalus atrox | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.44 | ion-exchange chromatography and gel filtration | Crotalus atrox |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.44 | venom | - |
Crotalus atrox | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.44 | basement membrane preperation from Engel-breth-Holm-Swarm tumor + H2O | great proteolytic activity | Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | collagen type IV + H2O | - |
Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | Dimethylcasein + H2O | - |
Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | Fibrinogen + H2O | rapid cleavage of the Aalpha chain followed by the Bbeta and gamma chains | Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | Laminin + H2O | - |
Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | additional information | no cleavage of fibrin | Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | Nidogen + H2O | - |
Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | o-aminobenzoyl-Ala-Gly-Leu-Ala-nitrobenzylamide + H2O | - |
Crotalus atrox | ? | - |
? | |
| 3.4.24.44 | Oxidized insulin B-chain + H2O | - |
Crotalus atrox | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.44 | monomer | - |
Crotalus atrox |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.44 | Hemorrhagic toxin e | - |
Crotalus atrox |
| 3.4.24.44 | HT-e | - |
Crotalus atrox |
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Release 2023.1 (January 2023)
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