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Literature summary extracted from
- Choriolysin H (2004), Handbook Of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 625-627.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.24.67 | degradation | HCE dissolves the inner layer of the egg envelope to facilitate hatching of the embryo | Oryzias latipes |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.67 | - |
Oryzias latipes |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.67 | antichoriolysin H | inhibits both choriolytic activity and binding to the egg envelope, does not affect caseinolytic activity | Oryzias latipes | |
| 3.4.24.67 | EDTA | inhibits caseinolytic or choriolytic activity at 1 mM | Oryzias latipes |  |
| 3.4.24.67 | additional information | not inhibited by DFP, iodoacetamide or iodoacetic acid | Oryzias latipes |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.67 | Ca2+ | - |
Oryzias latipes | |
| 3.4.24.67 | Cu2+ | high concentrations reactivate EDTA-inactivated apoenzyme | Oryzias latipes | |
| 3.4.24.67 | Mg2+ | - |
Oryzias latipes | |
| 3.4.24.67 | Zn2+ | low concentrations reactivate EDTA-inactivated apoenzyme | Oryzias latipes | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.67 | 24000 | - |
- |
Oryzias latipes |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.67 | Oryzias latipes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.67 | partly purified by cation-exchange column chromatography and gel filtration, two isoforms | Oryzias latipes |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.67 | embryo | expression of the enzyme starts early in the embryonic development, i. e. at the late gastrula stage | Oryzias latipes | - |
| 3.4.24.67 | hatching gland | colocalized with choriolysin L in the secretory granules | Oryzias latipes | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.67 | casein + H2O | - |
Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | chorion + H2O | - |
Oryzias latipes | ? | - |
? | |
| 3.4.24.67 | Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O | - |
Oryzias latipes | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.67 | monomer | - |
Oryzias latipes |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.67 | chorionase | - |
Oryzias latipes |
| 3.4.24.67 | HCE | - |
Oryzias latipes |
| 3.4.24.67 | High choriolytic enzyme | - |
Oryzias latipes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.67 | 8 | 8.7 | for choriolytic and caseinolytic activity, respectively | Oryzias latipes |
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Release 2023.1 (January 2023)
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