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Literature summary extracted from

  • Ishii, S.; Kumazaki, T.
    Mycolysin (2004), Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 412-413.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.31 expressed in Streptomyces lividans Streptomyces cacaoi

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.31 additional information mutations in the hydrophobic region or in the proline at the boundary of the hydrophobic core-cleavage region delay export of the enzyme and reduce total secretion by a factor of 2-10 Streptomyces cacaoi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.31 EDTA 10 mM inactivates activity almost completely, addition of 1 mM Zn2+, Co2+ or Mn2+ can restore depressed activity to 100, 142 and 115% respectively Streptomyces cacaoi
3.4.24.31 additional information not inhibited by PMSF and iodoacetamide Streptomyces cacaoi

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.31 Streptomyces cacaoi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.31 by acetone precipitation and Fractogel CM-650 chromatography Streptomyces cacaoi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.31 azocasein + H2O
-
 Streptomyces cacaoi ?
-
 ?
3.4.24.31 casein + H2O
-
 Streptomyces cacaoi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.31 monomer
-
 Streptomyces cacaoi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.31 5 7
-
 Streptomyces cacaoi