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Literature summary extracted from

  • Fricker, L.D.
    Carboxypeptidase E (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ) Academic Press, 2, 840-844.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.10 DNA and amino acid sequence determination, localization on chromosome 4, several naturally occurring genetic variants exist Homo sapiens
3.4.17.10 DNA and amino acid sequence determination, localization on chromosome 8 Mus musculus
3.4.17.10 high level expression in insect cells using the baculovirus transfection system, the recombinant enzyme is indistinguishable from the native enzyme, expression in bacteria results in inactive enzyme Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.17.10 R283W naturally occurring genetic variant of the enzyme, the mutant shows altered kinetics, heat stability, and pH optimum, and a 50% reduced activity compared to the wild-type enzyme Homo sapiens
3.4.17.10 S202P enzyme-defective mutant CPEfat/fat mice show a point mutation in the coding region of the gene encoding the enzyme, and lack enzyme activity completely, which affects peptide processing the mutant mice Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.10 1,10-phenanthroline
-
 Aplysia sp.
3.4.17.10 1,10-phenanthroline
-
 Bos taurus
3.4.17.10 1,10-phenanthroline
-
 Homo sapiens
3.4.17.10 1,10-phenanthroline
-
 Mus musculus
3.4.17.10 1,10-phenanthroline
-
 Rattus norvegicus
3.4.17.10 4-Chloromercuriphenylsulfonate over 95% inhibition at 0.1 mM Bos taurus
3.4.17.10 HgCl2
-
 Aplysia sp.
3.4.17.10 HgCl2 over 95% inhibition at 0.01 mM Bos taurus
3.4.17.10 HgCl2
-
 Homo sapiens
3.4.17.10 HgCl2
-
 Mus musculus
3.4.17.10 HgCl2
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.17.10 extracellular secretion from neuroendocrine tissue Mus musculus
-
-
3.4.17.10 extracellular secretion from neuroendocrine tissue Homo sapiens
-
-
3.4.17.10 extracellular secretion from neuroendocrine tissue Rattus norvegicus
-
-
3.4.17.10 extracellular secretion from neuroendocrine tissue Bos taurus
-
-
3.4.17.10 extracellular secretion from neuroendocrine tissue Aplysia sp.
-
-
3.4.17.10 membrane
-
 Bos taurus 16020
-
3.4.17.10 membrane the enzyme is sorted into the secretory pathway via its C-terminal domain Mus musculus 16020
-
3.4.17.10 membrane the enzyme is sorted into the secretory pathway via its C-terminal domain Homo sapiens 16020
-
3.4.17.10 membrane the enzyme is sorted into the secretory pathway via its C-terminal domain Rattus norvegicus 16020
-
3.4.17.10 membrane the enzyme is sorted into the secretory pathway via its C-terminal domain Aplysia sp. 16020
-
3.4.17.10 vesicle enkephalin-containing chromaffin vesicles in the adrenal medulla, the enzyme is sorted into the secretory pathway via its C-terminal domain Bos taurus 31982
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.10 Co2+ stimulates in the millimolar concentration range Mus musculus
3.4.17.10 Co2+ stimulates in the millimolar concentration range Homo sapiens
3.4.17.10 Co2+ stimulates in the millimolar concentration range Rattus norvegicus
3.4.17.10 Co2+ stimulates in the millimolar concentration range Bos taurus
3.4.17.10 Co2+ stimulates in the millimolar concentration range Aplysia sp.
3.4.17.10 additional information metallocarboxypeptidase Mus musculus
3.4.17.10 additional information metallocarboxypeptidase Homo sapiens
3.4.17.10 additional information metallocarboxypeptidase Rattus norvegicus
3.4.17.10 additional information metallocarboxypeptidase Bos taurus
3.4.17.10 additional information metallocarboxypeptidase Aplysia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.10 additional information Aplysia sp. the enzyme contributes to the sorting of proteins into the secretory pathway ?
-
 ?
3.4.17.10 additional information Mus musculus the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters ?
-
 ?
3.4.17.10 additional information Homo sapiens the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters ?
-
 ?
3.4.17.10 additional information Rattus norvegicus the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters ?
-
 ?
3.4.17.10 additional information Bos taurus the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.10 Aplysia sp.
-
-
-
3.4.17.10 Bos taurus
-
-
-
3.4.17.10 Homo sapiens
-
 different genetic variants
-
3.4.17.10 Mus musculus
-
-
-
3.4.17.10 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.17.10 glycoprotein glycosylation does not appear to affect the enzyme activity Mus musculus
3.4.17.10 glycoprotein glycosylation does not appear to affect the enzyme activity Homo sapiens
3.4.17.10 glycoprotein glycosylation does not appear to affect the enzyme activity Rattus norvegicus
3.4.17.10 glycoprotein glycosylation does not appear to affect the enzyme activity Bos taurus
3.4.17.10 glycoprotein glycosylation does not appear to affect the enzyme activity Aplysia sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.10 native enzyme from brain to homogeneity by 4-aminobenzoyl affinity chromatography Rattus norvegicus
3.4.17.10 native enzyme from pituitary to homogeneity from pituitary gland by 4-aminobenzoyl-Arg affinity chromatography with elution at pH 8.0 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.10 adrenal medulla
-
 Bos taurus
-
3.4.17.10 brain
-
 Rattus norvegicus
-
3.4.17.10 neuroendocrine cell
-
 Mus musculus
-
3.4.17.10 neuroendocrine cell
-
 Homo sapiens
-
3.4.17.10 neuroendocrine cell
-
 Rattus norvegicus
-
3.4.17.10 neuroendocrine cell
-
 Bos taurus
-
3.4.17.10 pancreas
-
 Mus musculus
-
3.4.17.10 pancreatic beta cell
-
 Mus musculus
-
3.4.17.10 pituitary gland
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.10 acetyl-Tyr-Ala-Arg + H2O
-
 Mus musculus acetyl-Tyr-Ala + Arg
-
 ?
3.4.17.10 acetyl-Tyr-Ala-Arg + H2O
-
 Homo sapiens acetyl-Tyr-Ala + Arg
-
 ?
3.4.17.10 acetyl-Tyr-Ala-Arg + H2O
-
 Rattus norvegicus acetyl-Tyr-Ala + Arg
-
 ?
3.4.17.10 acetyl-Tyr-Ala-Arg + H2O
-
 Bos taurus acetyl-Tyr-Ala + Arg
-
 ?
3.4.17.10 acetyl-Tyr-Ala-Arg + H2O
-
 Aplysia sp. acetyl-Tyr-Ala + Arg
-
 ?
3.4.17.10 benzoyl-Phe-Ala-Arg + H2O
-
 Mus musculus benzoyl-Phe-Ala + Arg
-
 ?
3.4.17.10 benzoyl-Phe-Ala-Arg + H2O
-
 Homo sapiens benzoyl-Phe-Ala + Arg
-
 ?
3.4.17.10 benzoyl-Phe-Ala-Arg + H2O
-
 Rattus norvegicus benzoyl-Phe-Ala + Arg
-
 ?
3.4.17.10 benzoyl-Phe-Ala-Arg + H2O
-
 Bos taurus benzoyl-Phe-Ala + Arg
-
 ?
3.4.17.10 benzoyl-Phe-Ala-Arg + H2O
-
 Aplysia sp. benzoyl-Phe-Ala + Arg
-
 ?
3.4.17.10 Dansyl-Phe-Ala-Arg + H2O
-
 Mus musculus Dansyl-Phe-Ala + Arg
-
 ?
3.4.17.10 Dansyl-Phe-Ala-Arg + H2O
-
 Homo sapiens Dansyl-Phe-Ala + Arg
-
 ?
3.4.17.10 Dansyl-Phe-Ala-Arg + H2O
-
 Rattus norvegicus Dansyl-Phe-Ala + Arg
-
 ?
3.4.17.10 Dansyl-Phe-Ala-Arg + H2O
-
 Bos taurus Dansyl-Phe-Ala + Arg
-
 ?
3.4.17.10 Dansyl-Phe-Ala-Arg + H2O
-
 Aplysia sp. Dansyl-Phe-Ala + Arg
-
 ?
3.4.17.10 additional information the enzyme contributes to the sorting of proteins into the secretory pathway Aplysia sp. ?
-
 ?
3.4.17.10 additional information the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters Mus musculus ?
-
 ?
3.4.17.10 additional information the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters Homo sapiens ?
-
 ?
3.4.17.10 additional information the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters Rattus norvegicus ?
-
 ?
3.4.17.10 additional information the enzyme contributes to the sorting of proteins into the secretory pathway, and is involved in the biosynthesis of several peptide hormones and neurotransmitters Bos taurus ?
-
 ?
3.4.17.10 additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Mus musculus ?
-
 ?
3.4.17.10 additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Homo sapiens ?
-
 ?
3.4.17.10 additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Rattus norvegicus ?
-
 ?
3.4.17.10 additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Bos taurus ?
-
 ?
3.4.17.10 additional information the enzyme removes C-terminal basic residues, Arg, Lys, and to a minor extent also His, from a variety of substrates, but shows no activity against nonbasic residues, the enzyme prefers Met at the P1 position but tolerates all amino acids, showing low activity in cleaving Pro-Arg bonds, it has a preference for tri- and tetrapeptides compared to dipeptides Aplysia sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.17.10 ? x * 52000-56000 Rattus norvegicus
3.4.17.10 ? x * 52000-56000 Bos taurus
3.4.17.10 More structure overview Mus musculus
3.4.17.10 More structure overview Homo sapiens
3.4.17.10 More structure overview Rattus norvegicus
3.4.17.10 More structure overview Bos taurus
3.4.17.10 More structure overview Aplysia sp.

Synonyms

EC Number Synonyms Comment Organism
3.4.17.10 carboxypeptidase H
-
 Bos taurus
3.4.17.10 CPE
-
 Mus musculus
3.4.17.10 CPE
-
 Homo sapiens
3.4.17.10 CPE
-
 Rattus norvegicus
3.4.17.10 CPE
-
 Bos taurus
3.4.17.10 CPE
-
 Aplysia sp.
3.4.17.10 enkephalin convertase formerly Bos taurus
3.4.17.10 More the enzyme belongs to the family of metallocarboxypeptidases Mus musculus
3.4.17.10 More the enzyme belongs to the family of metallocarboxypeptidases Homo sapiens
3.4.17.10 More the enzyme belongs to the family of metallocarboxypeptidases Rattus norvegicus
3.4.17.10 More the enzyme belongs to the family of metallocarboxypeptidases Bos taurus
3.4.17.10 More the enzyme belongs to the family of metallocarboxypeptidases Aplysia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.10 5 5.5
-
 Mus musculus
3.4.17.10 5 5.5
-
 Homo sapiens
3.4.17.10 5 5.5
-
 Rattus norvegicus
3.4.17.10 5 5.5
-
 Bos taurus
3.4.17.10 5 5.5
-
 Aplysia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.17.10 additional information
-
 rapid decline in activity at pH values above pH 5.5 Mus musculus
3.4.17.10 additional information
-
 rapid decline in activity at pH values above pH 5.5 Homo sapiens
3.4.17.10 additional information
-
 rapid decline in activity at pH values above pH 5.5 Rattus norvegicus
3.4.17.10 additional information
-
 rapid decline in activity at pH values above pH 5.5 Bos taurus
3.4.17.10 additional information
-
 rapid decline in activity at pH values above pH 5.5 Aplysia sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.17.10 Mus musculus about
-
 5
3.4.17.10 Homo sapiens about
-
 5
3.4.17.10 Rattus norvegicus about
-
 5
3.4.17.10 Bos taurus about
-
 5
3.4.17.10 Aplysia sp. about
-
 5