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Literature summary extracted from

  • Scholze, H.; Tannich, E.
    Histolysain and other Entamoeba cysteine endopeptidases (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2, 1164-1166.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.35 expression of partial gene ehcp1 in Escherichia coli as GST-fusion protein, expression of functional EhCP2 in insect cells using the baculovirus transfection system Entamoeba histolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.35 cell surface the enzyme is secreted and localized on the cell surface Entamoeba histolytica 9986
-
3.4.22.35 lysosome lysosome-like vesicles Entamoeba histolytica 5764
-
3.4.22.35 membrane the enzyme contains a transmembrane segment Entamoeba histolytica 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.35 27000
-
 1 * 27000, about Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.35 additional information Entamoeba histolytica the enzyme degrades a number of proteins, including those of the extracellular matrix ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.35 Entamoeba dispar
-
 gene edcp2 or acp2
-
3.4.22.35 Entamoeba histolytica
-
 gene ehcp2 or acp2 encodes histolysain, gene ehcp1 or acp1 encodes amoebapain
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.35 no glycoprotein no N-glycosylation Entamoeba histolytica

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.35 to homogeneity Entamoeba histolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.35 additional information the enzyme is expressed and active in different life cycle stages Entamoeba histolytica
-
3.4.22.35 trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.35 Arg-Gly-Phe-Phe + H2O substrate of amoebapain Entamoeba histolytica Arg-Gly + Phe-Phe
-
 ?
3.4.22.35 azocasein + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 Azocoll + H2O
-
 Entamoeba histolytica ?
-
 ?
3.4.22.35 additional information the enzyme degrades a number of proteins, including those of the extracellular matrix Entamoeba histolytica ?
-
 ?
3.4.22.35 additional information the enzyme degrades a number of proteins, including those of the extracellular matrix, but also acts on small molecule substrates showing endopeptidase and exopeptidase-like activities, amoebapain hydrolyzes unblocked tetrapeptides with basic residues at P2 Entamoeba histolytica ?
-
 ?
3.4.22.35 Z-Arg-Arg-4-methoxy-2-nitroanilide + H2O
-
 Entamoeba histolytica Z-Arg-Arg + 4-methoxy-2-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.35 monomer 1 * 27000, about Entamoeba histolytica
3.4.22.35 More the enzyme contains an RGD integrin attachment motif, structure-function overview Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
3.4.22.35 amoebapain
-
 Entamoeba histolytica
3.4.22.35 EhCP1
-
 Entamoeba histolytica
3.4.22.35 EhCP2
-
 Entamoeba histolytica
3.4.22.35 Histolysin
-
 Entamoeba histolytica
3.4.22.35 Histolysin
-
 Entamoeba dispar
3.4.22.35 More the enzyme belongs to the C1 peptidase family Entamoeba histolytica
3.4.22.35 More the enzyme belongs to the C1 peptidase family Entamoeba dispar

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.35 5 6 dependent on the substrate Entamoeba histolytica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.35 Entamoeba histolytica
-
-
 additional information