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Literature summary extracted from

  • Buttle, D.J.
    Glycyl endopeptidase (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2, 1132-1134.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.25 additional information the enzyme depends on a thiolate ion for activity Carica papaya

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.25 G27E site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position Carica papaya
3.4.22.25 G73R site-directed mutagenesis, high reduced activity with substrate having an Arg at P1 position, decreased affinity for cystatin C Carica papaya

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.25 Alpha-macroglobulin
-
 Carica papaya
3.4.22.25 E64 inactivation Carica papaya
3.4.22.25 iodoacetamide slow inactivation Carica papaya
3.4.22.25 iodoacetate slow inactivation Carica papaya
3.4.22.25 additional information no inhibition by most cystatins Carica papaya
3.4.22.25 papaya cystatin
-
 Carica papaya
3.4.22.25 Peptidyl diazomethanes dependent on the substrate Carica papaya
3.4.22.25 Pro-peptide the enzyme is inhibited by its own pro region after cleavage, also pro-peptide of other C1 peptidase family enzymes can be inhibitory Carica papaya

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.25 extracellular latex Carica papaya
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.25 23313
-
 1 * 23313 Carica papaya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.25 additional information Carica papaya the enzyme is useful for the plant defense system ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.25 Carica papaya
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.25 no glycoprotein mature enzyme Carica papaya
3.4.22.25 proteolytic modification the enzyme is synthesized as preproenzyme Carica papaya

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.25 different methods, overview Carica papaya

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.25 latex
-
 Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.25 Chicken cystatin + H2O
-
 Carica papaya ?
-
 ?
3.4.22.25 Gly-4-nitroanilide + H2O
-
 Carica papaya glycine + 4-nitroaniline
-
 ?
3.4.22.25 Human cystatin C + H2O
-
 Carica papaya ?
-
 ?
3.4.22.25 additional information the enzyme is useful for the plant defense system Carica papaya ?
-
 ?
3.4.22.25 additional information substrate specificity with preference for glycyl residues, structure-function relationship, overview, hydrolyzes glycine esters but not Bz-Arg-4-nitroanilide Carica papaya ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.25 monomer 1 * 23313 Carica papaya
3.4.22.25 More three-dimensional structure comparison to other latex peptidase Carica papaya

Synonyms

EC Number Synonyms Comment Organism
3.4.22.25 Chymopapain M
-
 Carica papaya
3.4.22.25 Glycyl endopeptidase
-
 Carica papaya
3.4.22.25 More the enzyme belongs to the C1A peptidase family Carica papaya
3.4.22.25 Papaya peptidase B
-
 Carica papaya
3.4.22.25 Papaya proteinase IV
-
 Carica papaya

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.25 7
-
 about Carica papaya

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.25 0.0000034
-
 papapya cystatin
-
 Carica papaya
3.4.22.25 0.00086
-
 Pro-peptide
-
 Carica papaya