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Literature summary extracted from

  • dEnfert, C.; Ibrahim-Granet, O.
    Saccharolysin (2004), Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press, 1, 360-361.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.4.24.37 degradation most likely major intracellular oligopeptidase responsible for the degradation of peptides resulting from nonvacuolar proteolysis Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.37
-
 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.37 additional information prd1-6 mutants with thermolabile saccharolysin activity, prd1::URA3 mutants devoid of the cytoplasmic and mitochondrial activities Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.37 4-hydroxymercuribenzoate 100% inhibition with 0.1 mM Saccharomyces cerevisiae
3.4.24.37 Cpp-Ala-Ala-Phe-p-aminobenzoic acid strongly inhibits Saccharomyces cerevisiae
3.4.24.37 EDTA inhibits 50% at 1 mM and 90% at 10 mM, can be efficiently reactivated by the addition of Zn2+, Co2+ and Mn2+ Saccharomyces cerevisiae
3.4.24.37 HgCl2 100% inhibition with 0.01 mM Saccharomyces cerevisiae
3.4.24.37 additional information not inhibited by DTT, N-ethylmaleimide, iodoacetic acid or iodoacetamide Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.37 0.006
-
 Pz-Pro-Leu-Gly-Pro-D-Arg
-
 Saccharomyces cerevisiae
3.4.24.37 0.029
-
 Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Saccharomyces cerevisiae
3.4.24.37 0.035
-
 MeOSuc-Ala-Ala-Pro-Met-NHPhNO2
-
 Saccharomyces cerevisiae
3.4.24.37 0.06
-
 Bz-Pro-Phe-Arg-NHPhNO2
-
 Saccharomyces cerevisiae
3.4.24.37 0.085
-
 Ac-Ala-Ala-Pro-Phe-NHPhNO2
-
 Saccharomyces cerevisiae
3.4.24.37 0.19
-
 Ac-Ala-Ala-Pro-Met-NHPhNO2
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.37 cytosol
-
 Saccharomyces cerevisiae 5829
-
3.4.24.37 mitochondrion intermembrane space Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.37 81800
-
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.37 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.37 250fold by chromatographies Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.37 Ac-Ala-Ala-Pro-Met-NHPhNO2 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.24.37 Ac-Ala-Ala-Pro-Phe-NHPhNO2 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.24.37 bradykinin + H2O cleaved at the Phe-Ser bond Saccharomyces cerevisiae ?
-
 ?
3.4.24.37 Bz-Pro-Phe-Arg-NHPhNO2 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.24.37 Dnp-Pro-Leu-Gly-Pro-Trp-D-Lys + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.24.37 Mcc-Pro-Leu-Gly-Pro-D-Lys(Dnp) + H2O
-
 Saccharomyces cerevisiae Mcc-Pro-Leu + Gly-Pro-D-Lys(Dnp)
-
 ?
3.4.24.37 MeOSuc-Ala-Ala-Pro-Met-NHPhNO2 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.24.37 additional information no cleavage of peptides with a free N-terminus, azocasein and (3H)methylcasein Saccharomyces cerevisiae ?
-
 ?
3.4.24.37 Pz-Pro-Leu-Gly-Pro-D-Arg + H2O
-
 Saccharomyces cerevisiae Pz-Pro-Leu + Gly-Pro-D-Arg
-
 ?
3.4.24.37 Pz-Pro-Leu-Gly-Pro-D-Arg + H2O
-
 Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.37 monomer
-
 Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.4.24.37 EC 3.4.22.22 formerly Saccharomyces cerevisiae
3.4.24.37 Prd1
-
 Saccharomyces cerevisiae
3.4.24.37 yscD
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.37 5.5
-
 with MeOSuc-Ala-Ala-Pro-Met-NHPhNO2 Saccharomyces cerevisiae
3.4.24.37 5.9
-
 with Ac-Ala-Ala-Pro-Met-NHPhNO2 Saccharomyces cerevisiae
3.4.24.37 7.9
-
 with Bz-Pro-Phe-Arg-NHPhNO2 Saccharomyces cerevisiae