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Literature summary extracted from

  • Kawakami, A.; Yoshida, M.; Van den Ende, W.
    Molecular cloning and functional analysis of a novel 6&1-FEH from wheat (Triticum aestivum L.) preferentially degrading small graminans like bifurcose (2005), Gene, 358, 93-101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 heterologous expression in Pichia pastoris X-33 Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 centrifugation, concentration and desaltation with 20 mM citrate-phosphate buffer pH 5.0 on Amicon Ultra-15 concentrator Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.80 epicotyl
-
 Triticum aestivum
-
3.2.1.80 additional information no activity in leaves Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 1,1,1-kestopentaose + H2O
-
 Triticum aestivum beta-D-fructose + ?
-
 ?
3.2.1.80 1,1-nystose + H2O
-
 Triticum aestivum beta-D-fructose + sucrose
-
 ?
3.2.1.80 1-kestose + H2O
-
 Triticum aestivum beta-D-fructose + sucrose
-
 ?
3.2.1.80 6-kestose + H2O
-
 Triticum aestivum beta-D-fructose + sucrose
-
 ?
3.2.1.80 levan + H2O
-
 Triticum aestivum beta-D-fructose + ?
-
 ?
3.2.1.80 additional information no activity towards sucrose and high degree of polymerization-inulin Triticum aestivum ?
-
 ?
3.2.1.80 wheat graminan + H2O high degree of polymerization Triticum aestivum beta-D-fructose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 6&1-FEH w1
-
 Triticum aestivum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.80 Triticum aestivum
-
-
 5.2