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Literature summary extracted from

  • Kanamori, T.; Kanou, N.; Kusakabe, S.; Atomi, H.; Imanaka, T.
    Allophanate hydrolase of Oleomonas sagaranensis involved in an ATP-dependent degradation pathway specific to urea (2005), FEMS Microbiol. Lett., 245, 61-65.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.54 overexpression in Escherichia coli strain BL21(DE3) Oleomonas sagaranensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.54 0.042
-
 allophanate pH 9.5, recombinant enzyme Oleomonas sagaranensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.54 61999
-
 2 * 61999, sequence calculation Oleomonas sagaranensis
3.5.1.54 138000
-
 gel filtration, recombinant enzyme Oleomonas sagaranensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.54 allophanate + H2O Oleomonas sagaranensis the enzyme forms an alternative urea degradation pathway with the urea carboxylase, EC 6.3.4.6 NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O Oleomonas sagaranensis HD-1 the enzyme forms an alternative urea degradation pathway with the urea carboxylase, EC 6.3.4.6 NH3 + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.54 Oleomonas sagaranensis
-
 isolated from an oil field in Sagara, Japan
-
3.5.1.54 Oleomonas sagaranensis HD-1
-
 isolated from an oil field in Sagara, Japan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.54 recombinant enzyme from Escherichia coli strain BL21(DE3) by ultracentrifucation, ion exchange chromatography, and gel filtration, 2.8fold to homogeneity Oleomonas sagaranensis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.54 urea-1-carboxylate + H2O = 2 CO2 + 2 NH3 the catalytic triad consists of residues Ser177, Ser153, and Lys79 Oleomonas sagaranensis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.54 108
-
 purified recombinant enzyme, substrate allophanate Oleomonas sagaranensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.54 allophanate + H2O
-
 Oleomonas sagaranensis NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme forms an alternative urea degradation pathway with the urea carboxylase, EC 6.3.4.6 Oleomonas sagaranensis NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O
-
 Oleomonas sagaranensis HD-1 NH3 + CO2
-
 ir
3.5.1.54 allophanate + H2O the enzyme forms an alternative urea degradation pathway with the urea carboxylase, EC 6.3.4.6 Oleomonas sagaranensis HD-1 NH3 + CO2
-
 ir

Subunits

EC Number Subunits Comment Organism
3.5.1.54 dimer 2 * 61999, sequence calculation Oleomonas sagaranensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.54 25
-
 assay at Oleomonas sagaranensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.54 7.5
-
 assay at Oleomonas sagaranensis