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Brucella abortus nicotinamidase (PncA) contributes to its intracellular replication and infectivity in mice

Kim, S.; Kurokawa, D.; Watanabe, K.; Makino, S.i.; Shirahata, T.; Watarai, M.; FEMS Microbiol. Lett. 234, 289-295 (2004)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
additional information
a gene disruption PncA mutant, a nicotinamidase/pyrazinamidase mutant, fails to replicate in HeLa cells and shows lower replication rates compared to the wild-type in macrophages, the mutant does not co-localize with host late endosomes or lysosomes, effects of gene disruption in vivo and in vitro, overview
Brucella abortus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Brucella abortus
contributes to the intracellular replication and infectivity of Brucella abortus in mice
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Brucella abortus 544 / ATCC 23448
contributes to the intracellular replication and infectivity of Brucella abortus in mice
nicotinate + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Brucella abortus
-
gene pncA, a facultative intracellular pathogen
-
3.5.1.19
Brucella abortus 544 / ATCC 23448
-
gene pncA, a facultative intracellular pathogen
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.19
additional information
the pathogen is cultured in human HeLa cells and in murine bone marrow macrophages, co-localizes with host late endosomes or lysosomes
Brucella abortus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
668655
Brucella abortus
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
contributes to the intracellular replication and infectivity of Brucella abortus in mice
668655
Brucella abortus
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
668655
Brucella abortus 544 / ATCC 23448
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
contributes to the intracellular replication and infectivity of Brucella abortus in mice
668655
Brucella abortus 544 / ATCC 23448
nicotinate + NH3
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
additional information
a gene disruption PncA mutant, a nicotinamidase/pyrazinamidase mutant, fails to replicate in HeLa cells and shows lower replication rates compared to the wild-type in macrophages, the mutant does not co-localize with host late endosomes or lysosomes, effects of gene disruption in vivo and in vitro, overview
Brucella abortus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Brucella abortus
contributes to the intracellular replication and infectivity of Brucella abortus in mice
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Brucella abortus 544 / ATCC 23448
contributes to the intracellular replication and infectivity of Brucella abortus in mice
nicotinate + NH3
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.19
additional information
the pathogen is cultured in human HeLa cells and in murine bone marrow macrophages, co-localizes with host late endosomes or lysosomes
Brucella abortus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
668655
Brucella abortus
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
contributes to the intracellular replication and infectivity of Brucella abortus in mice
668655
Brucella abortus
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
-
668655
Brucella abortus 544 / ATCC 23448
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
contributes to the intracellular replication and infectivity of Brucella abortus in mice
668655
Brucella abortus 544 / ATCC 23448
nicotinate + NH3
-
-
-
?