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Literature summary extracted from

  • Lukacin, R.; Matern, U.; Specker, S.; Vogt, T.
    Cations modulate the substrate specificity of bifunctional class I O-methyltransferase from Ammi majus (2004), FEBS Lett., 577, 367-370.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.104 expression in Escherichia coli Ammi majus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.104 Cu2+ 0.15 mM, completely abolishes the rate of methylation of caffeoyl-CoA Ammi majus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.104 0.0041
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
2.1.1.104 0.009
-
 quercetagetin pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
2.1.1.104 0.02
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.031
-
 quercetin pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
2.1.1.104 0.039
-
 3,4-dihydroxybenzoic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.061
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.068
-
 5-hydroxyferulic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.073
-
 quercetin pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.088
-
 caffeic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.93
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.104 Co2+ 0.15 mM, about 2fold increase of the rate of methylation of caffeoyl-CoA. Replacing Co2+ for Mg2+ drastically shifts the substrate preferences towards caffeic, 5-hydroxyferulic and 3,4-dihydroxybenzoic acid, which are not at all accepted in assays with Mg2+ as cofactor Ammi majus
2.1.1.104 Mg2+ required Ammi majus
2.1.1.104 Mn2+ 0.15 mM, about 2fold increase of the rate of methylation of caffeoyl-CoA. Replacing Mn2+ for Mg2+ drastically shifts the substrate preferences towards caffeic, 5-hydroxyferulic and 3,4-dihydroxybenzoic acid, which are not at all accepted in assays with Mg2+ as cofactor Ammi majus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.104 27000
-
 x * 27000, SDS-PAGE Ammi majus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.104 Ammi majus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.104 recombinant enzyme Ammi majus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.104 culture condition:dark-grown cell
-
 Ammi majus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.104 S-adenosyl-L-methionine + 3,4-dihydroxybenzoic acid activity in presence of Co2+, no activity in presence of Mg2+ Ammi majus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxy-ferulic acid activity in presence of Co2+, no activity in presence of Mg2+ Ammi majus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeic acid activity in presence of Co2+, no activity in presence of Mg2+ Ammi majus S-adenosyl-L-homocysteine + ferulic acid
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Ammi majus S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-D-glucose
-
 Ammi majus S-adenosyl-L-homocysteine + feruloyl-D-glucose
-
 ?
2.1.1.104 S-adenosyl-L-methionine + quercetagetin no activity in presence of Co2+, low activity in presence of Mg2+ Ammi majus S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + quercetin
-
 Ammi majus S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.104 ? x * 27000, SDS-PAGE Ammi majus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.104 caffeoyl-coenzyme A O-methyltransferase
-
 Ammi majus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.104 0.013
-
 3,4-dihydroxybenzoic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.068
-
 caffeic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.071
-
 quercetagetin pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
2.1.1.104 0.143
-
 5-hydroxyferulic acid pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.149
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
2.1.1.104 0.206
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
2.1.1.104 0.268
-
 quercetin pH 7.5, 37°C, in presence of 0.15 mM Mg2+ Ammi majus
2.1.1.104 0.631
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 0.938
-
 quercetin pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 1.043
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 6.08
-
 quercetin pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 6.08
-
 caffeoyl-CoA pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus
2.1.1.104 6.08
-
 caffeoyl-D-glucose pH 7.5, 37°C, in presence of 0.1 mM Co2+ Ammi majus