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Literature summary extracted from

  • Canaan, S.; Maurin, D.; Chahinian, H.; Pouilly, B.; Durousseau, C.; Frassinetti, F.; Scappuccini-Calvo, L.; Cambillau, C.; Bourne, Y.
    Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis. A novel carboxyl esterase structurally related to the HSL family (2004), Eur. J. Biochem., 271, 3953-3961.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 diethyl 4-nitrophenyl phosphate i.e. E600, strong inhibitor Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 31700
-
 x * 31700, calculated Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Mycobacterium tuberculosis
-
 protein Rv1399c, overexpressed in Escherichia coli
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.1 a carboxylic ester + H2O = an alcohol + a carboxylate catalytic triad consists of S162, H290, and D260 and is located at the bottom of a solvent accessible pocket. The guanidine moiety close to H290 confers a low pH shift of the catalytic His molecule Mycobacterium tuberculosis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 184
-
 substrate triacetin, pH 7.0, 25°C Mycobacterium tuberculosis
3.1.1.1 456
-
 substrate tributyrine, pH 7.0, 25°C Mycobacterium tuberculosis
3.1.1.1 610
-
 substrate vinyl acetate, pH 7.0, 25°C Mycobacterium tuberculosis
3.1.1.1 1123
-
 substrate vinyl butanoate, pH 7.0, 25°C Mycobacterium tuberculosis
3.1.1.1 1350
-
 substrate tripropionin, pH 7.0, 25°C Mycobacterium tuberculosis
3.1.1.1 1618
-
 substrate vinyl propionate, pH 7.0, 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 triacetin + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
3.1.1.1 tributyrin + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
3.1.1.1 tripropionin + H2O
-
 Mycobacterium tuberculosis ?
-
 ?
3.1.1.1 vinyl acetate + H2O
-
 Mycobacterium tuberculosis vinyl alcohol + acetate
-
 ?
3.1.1.1 vinyl butanoate + H2O
-
 Mycobacterium tuberculosis vinyl alcohol + butanoate
-
 ?
3.1.1.1 vinyl propionate + H2O
-
 Mycobacterium tuberculosis vinyl alcohol + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 31700, calculated Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 45
-
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 60
-
 no enzymic activity above Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7 9 broad Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.1 0.000003
-
 diethyl 4-nitrophenyl phosphate pH 7.0, 25°C Mycobacterium tuberculosis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Mycobacterium tuberculosis calculated
-
 4.53