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Literature summary extracted from

  • Shin, H.D.; Chen, R.R.
    Production and characterization of a type B feruloyl esterase from Fusarium proliferatum NRRL 26517 (2006), Enzyme Microb. Technol., 38, 478-485.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.146
-
 methyl ferulate pH 7, 40°C Fusarium proliferatum
3.1.1.73 0.196
-
 methyl caffeate pH 7, 40°C Fusarium proliferatum
3.1.1.73 0.263
-
 methyl p-coumarate pH 7, 40°C Fusarium proliferatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Fusarium proliferatum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 31000
-
 x * 31000, SDS-PAGE Fusarium proliferatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 corn bran + H2O Fusarium proliferatum crude enzyme preparation from the strain supplemented with a commercial xylanase can release about 32% of the alkaline-extractable ferulic acid from corn bran ferulic acid + ?
-
 ?
3.1.1.73 corn bran + H2O Fusarium proliferatum NRRL 26517 crude enzyme preparation from the strain supplemented with a commercial xylanase can release about 32% of the alkaline-extractable ferulic acid from corn bran ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O Fusarium proliferatum involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O Fusarium proliferatum NRRL 26517 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Fusarium proliferatum
-
 feruloyl esterase type B, inorganic nitrogen salt and an esterified ferulic acid in the culture medium required for enzyme production, complex organic nitrogen sources suppress enzyme production
-
3.1.1.73 Fusarium proliferatum NRRL 26517
-
 feruloyl esterase type B, inorganic nitrogen salt and an esterified ferulic acid in the culture medium required for enzyme production, complex organic nitrogen sources suppress enzyme production
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73
-
 Fusarium proliferatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 2.89
-
 purified enzyme, pH 7, 40°C Fusarium proliferatum
3.1.1.73 3.8
-
 methyl ferulate as substrate, pH 7, 40°C Fusarium proliferatum
3.1.1.73 9.6
-
 methyl p-coumarate as substrate, pH 7, 40°C Fusarium proliferatum
3.1.1.73 13.1
-
 methyl caffeate as substrate, pH 7, 40°C Fusarium proliferatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 corn bran + H2O crude enzyme preparation from the strain supplemented with a commercial xylanase can release about 32% of the alkaline-extractable ferulic acid from corn bran Fusarium proliferatum ferulic acid + ?
-
 ?
3.1.1.73 corn bran + H2O crude enzyme preparation from the strain supplemented with a commercial xylanase can release about 32% of the alkaline-extractable ferulic acid from corn bran Fusarium proliferatum NRRL 26517 ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Fusarium proliferatum ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Fusarium proliferatum NRRL 26517 ferulic acid + ?
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Fusarium proliferatum caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Fusarium proliferatum NRRL 26517 caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Fusarium proliferatum ferulic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Fusarium proliferatum NRRL 26517 ferulic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Fusarium proliferatum p-coumaric acid + methanol
-
 ?
3.1.1.73 additional information no activity with methyl sinapinate Fusarium proliferatum ?
-
 ?
3.1.1.73 additional information no activity with methyl sinapinate Fusarium proliferatum NRRL 26517 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 31000, SDS-PAGE Fusarium proliferatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
-
 Fusarium proliferatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 50
-
 unstable above 50°C Fusarium proliferatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6.5 7.5
-
 Fusarium proliferatum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 5
-
 enzyme retains over 80% of its activity at pH 5.0 after storage at for 12 h at 4°C Fusarium proliferatum
3.1.1.73 6 8 stable at pHs between 6 and 8 Fusarium proliferatum
3.1.1.73 9
-
 enzyme retains over 80% of its activity at pH 9.0 after storage at for 12 h at 4°C Fusarium proliferatum