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Literature summary extracted from

  • Cornista, J.; Ikeuchi, S.; Haruki, M.; Kohara, A.; Takano, K.; Morikawa, M.; Kanaya, S.
    Cleavage of various peptides with pitrilysin from Escherichia coli: kinetic analyses using beta-endorphin and its derivatives (2004), Biosci. Biotechnol. Biochem., 68, 2128-2137.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.55 0.00036
-
 beta-endorphin pH 8.0, 37°C Escherichia coli
3.4.24.55 0.0004
-
 Vasoactive intestinal peptide pH 8.0, 37°C Escherichia coli
3.4.24.55 0.0009
-
 beta-amyloid pH 8.0, 37°C Escherichia coli
3.4.24.55 0.0015
-
 Insulin B-chain pH 8.0, 37°C Escherichia coli
3.4.24.55 0.0022
-
 calcitonin pH 8.0, 37°C Escherichia coli
3.4.24.55 0.031
-
 Substance P pH 8.0, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.55 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.55 beta-amyloid + H2O 18% of the activity with beta-endorphin, major cleavage site His14-Gln15 Escherichia coli ?
-
 ?
3.4.24.55 beta-endorphin + H2O cleavage at Lys19-Asn20 bond. Residues Leu14, Val15 and Leu17 and region 22-26 are responsible for recognition by enzyme Escherichia coli ?
-
 ?
3.4.24.55 calcitonin + H2O 3% of the activity with beta-endorphin Escherichia coli ?
-
 ?
3.4.24.55 Glucagon + H2O 8% of the activity with beta-endorphin Escherichia coli ?
-
 ?
3.4.24.55 Insulin B-chain + H2O 17% of the activity with beta-endorphin, major cleavage site Tyr16-Leu17 Escherichia coli ?
-
 ?
3.4.24.55 Substance P + H2O 10% of the activity with beta-endorphin Escherichia coli ?
-
 ?
3.4.24.55 Vasoactive intestinal peptide + H2O 7.2% of the activity with beta-endorphin, major cleavage site Arg14-Lys15 Escherichia coli ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.55 0.43
-
 calcitonin pH 8.0, 37°C Escherichia coli
3.4.24.55 0.63
-
 Substance P pH 8.0, 37°C Escherichia coli
3.4.24.55 0.9
-
 Vasoactive intestinal peptide pH 8.0, 37°C Escherichia coli
3.4.24.55 2.33
-
 beta-amyloid pH 8.0, 37°C Escherichia coli
3.4.24.55 2.66
-
 Insulin B-chain pH 8.0, 37°C Escherichia coli
3.4.24.55 12.5
-
 beta-endorphin pH 8.0, 37°C Escherichia coli