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Literature summary extracted from

  • Li, Y.; Raushel, F.M.
    Inhibitors designed for the active site of dihydroorotase (2005), Bioorg. Chem., 33, 470-483.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.3 C221S/C263S/C265S/C268S this quadruple mutant is relatively stable to oxidation and has kinetic parameters consistent with reported values for wild type DHO Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.3 (S)-1,2,3,6-tetrahydro-6-oxopyridine-2-carboxylic acid competitive inhibitor versus dihydroorotate and thio-dihydroorotate Escherichia coli
3.5.2.3 4(R)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid competitive inhibitor versus dihydroorotate and thio-dihydroorotate Escherichia coli
3.5.2.3 4(S)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid competitive inhibitor versus dihydroorotate and thio-dihydroorotate Escherichia coli
3.5.2.3 4,6-dioxo-piperidine-2-(S)-carboxylic acid most active competitive inhibitor versus dihydroorotate and thio-dihydroorotate Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.3
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.3 L-dihydroorotate + H2O
-
 Escherichia coli N-carbamoyl-L-aspartate
-
 r
3.5.2.3 thio-dihydroorotate + H2O
-
 Escherichia coli ?
-
 r

Synonyms

EC Number Synonyms Comment Organism
3.5.2.3 DHO
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.3 0.076
-
 4,6-dioxo-piperidine-2-(S)-carboxylic acid with dihydroorotate as substrate, pH. 7.0 Escherichia coli
3.5.2.3 0.096
-
 4,6-dioxo-piperidine-2-(S)-carboxylic acid with thio-dihydroorotate as substrate, pH. 7.0 Escherichia coli
3.5.2.3 0.12
-
 4,6-dioxo-piperidine-2-(S)-carboxylic acid with thio-dihydroorotate as substrate, pH. 8.0 Escherichia coli
3.5.2.3 0.21
-
 4,6-dioxo-piperidine-2-(S)-carboxylic acid with dihydroorotate as substrate, pH. 8.0 Escherichia coli
3.5.2.3 0.32
-
 4,6-dioxo-piperidine-2-(S)-carboxylic acid with thio-dihydroorotate as substrate, pH. 9.0 Escherichia coli
3.5.2.3 0.44
-
 4,6-dioxo-piperidine-2-(S)-carboxylic acid with dihydroorotate as substrate, pH. 9.0 Escherichia coli
3.5.2.3 1.6
-
 4(R)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid with thio-dihydroorotate as substrate, pH. 8.0 Escherichia coli
3.5.2.3 2.3
-
 (S)-1,2,3,6-tetrahydro-6-oxopyridine-2-carboxylic acid with thio-dihydroorotate as substrate, pH. 8.0 Escherichia coli
3.5.2.3 3
-
 4(S)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid with thio-dihydroorotate as substrate, pH. 8.0 Escherichia coli