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Literature summary extracted from
- A preliminary account of the properties of recombinant human glyoxylate reductase (GRHPR), LDHA and LDHB with glyoxylate, and their potential roles in its metabolism (2005), Biochim. Biophys. Acta, 1753, 209-216.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.79 | expression in Escherichia coli strain JM109 | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.79 | additional information | - |
additional information | kinetics | Homo sapiens | |
| 1.1.1.79 | 0.011 | - |
NADPH | 37°C | Homo sapiens |  |
| 1.1.1.79 | 0.011 | - |
NADPH | pH 7.5, 37°C, recombinant enzyme | Homo sapiens | |
| 1.1.1.79 | 0.058 | - |
Hydroxypyruvate | 37°C, cofactor: NAPDH | Homo sapiens | |
| 1.1.1.79 | 0.058 | - |
Hydroxypyruvate | pH 7.5, 37°C, recombinant enzyme, with NADPH | Homo sapiens | |
| 1.1.1.79 | 0.19 | - |
Hydroxypyruvate | 37°C, cofactor: NADH | Homo sapiens | |
| 1.1.1.79 | 0.19 | - |
Hydroxypyruvate | pH 7.5, 37°C, recombinant enzyme, with NADH | Homo sapiens | |
| 1.1.1.79 | 0.24 | - |
glyoxylate | 37°C, cofactor: NADPH | Homo sapiens | |
| 1.1.1.79 | 0.24 | - |
glyoxylate | pH 7.5, 37°C, recombinant enzyme, with NADPH | Homo sapiens | |
| 1.1.1.79 | 1 | - |
glyoxylate | 37°C, cofactor: NADH | Homo sapiens | |
| 1.1.1.79 | 1 | - |
glyoxylate | pH 7.5, 37°C, recombinant enzyme, with NADH | Homo sapiens | |
| 1.1.1.79 | 2.42 | - |
NADH | 37°C | Homo sapiens | |
| 1.1.1.79 | 2.42 | - |
NADH | pH 7.5, 37°C, recombinant enzyme | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.79 | cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.79 | glyoxylate + NAD(P)H | Homo sapiens | the enzyme plays a protective role in detoxification of glyoxylate | glycolate + NAD(P)+ | - |
? | |
| 1.1.1.79 | hydroxypyruvate + NAD(P)H | Homo sapiens | - |
D-glycerate + NAD(P)+ + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.79 | Homo sapiens | - |
- |
- |
| 1.1.1.79 | Homo sapiens | - |
recombinant | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.79 | additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.79 | glyoxylate + NAD(P)H | - |
Homo sapiens | glycolate + NAD(P)+ | - |
? | |
| 1.1.1.79 | glyoxylate + NAD(P)H | the enzyme plays a protective role in detoxification of glyoxylate | Homo sapiens | glycolate + NAD(P)+ | - |
? | |
| 1.1.1.79 | glyoxylate + NADH | affinity for NADPH is lower than affinity for NADH | Homo sapiens | glycolate + NAD+ | - |
? | |
| 1.1.1.79 | glyoxylate + NADPH | - |
Homo sapiens | glycolate + NADP+ | - |
? | |
| 1.1.1.79 | hydroxypyruvate + NAD(P)H | - |
Homo sapiens | D-glycerate + NAD(P)+ + H+ | - |
? | |
| 1.1.1.79 | hydroxypyruvate + NAD(P)H | preferred substrate | Homo sapiens | D-glycerate + NAD(P)+ + H+ | - |
? | |
| 1.1.1.79 | hydroxypyruvate + NADH | affinity for NADPH is lower than affinity for NADH | Homo sapiens | D-glycerate + NAD+ | - |
? | |
| 1.1.1.79 | hydroxypyruvate + NADPH | - |
Homo sapiens | D-glycerate + NADP+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.79 | glyoxylate reductase | - |
Homo sapiens |
| 1.1.1.79 | GR/HPR | - |
Homo sapiens |
| 1.1.1.79 | GRHPR | - |
Homo sapiens |
| 1.1.1.79 | More | the enzyme shows bifunctionality also performing the reaction of hydroxypyruvate reductase, EC 1.1.1.81 | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.79 | 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.79 | 27 | - |
glyoxylate | 37°C, cofactor: NADPH | Homo sapiens | |
| 1.1.1.79 | 27 | - |
glyoxylate | pH 7.5, 37°C, recombinant enzyme, with NADPH | Homo sapiens | |
| 1.1.1.79 | 38 | - |
Hydroxypyruvate | 37°C, cofactor: NAPDH | Homo sapiens | |
| 1.1.1.79 | 38 | - |
Hydroxypyruvate | pH 7.5, 37°C, recombinant enzyme, with NADPH | Homo sapiens | |
| 1.1.1.79 | 65 | - |
Hydroxypyruvate | 37°C, cofactor: NADH | Homo sapiens | |
| 1.1.1.79 | 65 | - |
Hydroxypyruvate | pH 7.5, 37°C, recombinant enzyme, with NADH | Homo sapiens | |
| 1.1.1.79 | 67 | - |
glyoxylate | 37°C, cofactor: NADH | Homo sapiens | |
| 1.1.1.79 | 67 | - |
glyoxylate | pH 7.5, 37°C, recombinant enzyme, with NADH | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.79 | 7.5 | - |
assay at | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.79 | NADH | - |
Homo sapiens | |
| 1.1.1.79 | NADH | higher affinity for NADPH | Homo sapiens | |
| 1.1.1.79 | NADPH | preferred cofactor | Homo sapiens | |
| 1.1.1.79 | NADPH | lower affinity for NADH | Homo sapiens | |
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Release 2023.1 (January 2023)
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