Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Koseki, T.; Takahashi, K.; Fushinobu, S.; Iefuji, H.; Iwano, K.; Hashizume, K.; Matsuzawa, H.
    Mutational analysis of a feruloyl esterase from Aspergillus awamori involved in substrate discrimination and pH dependence (2005), Biochim. Biophys. Acta, 1722, 200-208.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73
-
 Aspergillus awamori

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 D77I higher catalytic efficiency towards alpha-naphtylbutyrate and alpha-naphtylcaprylate, some activity towards long-acyl chain esters, no activity towards phenolic acid methyl esters and feruloylated arabinoxylan, increased pH-optimum Aspergillus awamori
3.1.1.73 D77N increased pH-optimum Aspergillus awamori
3.1.1.73 L74W no enzyme protein detected Aspergillus awamori
3.1.1.73 S172A inactive Aspergillus awamori
3.1.1.73 T72R minor effect on activity Aspergillus awamori
3.1.1.73 Y80F higher catalytic efficiency towards alpha-naphtylbutyrate and alpha-naphtylcaprylate, increased pH-optimum Aspergillus awamori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.017
-
 alpha-naphthylcaprylate pH 5.0, 37°C, T72R mutant enzyme Aspergillus awamori
3.1.1.73 0.02
-
 alpha-naphthylcaprylate pH 5.0, 37°C, Y80F mutant enzyme Aspergillus awamori
3.1.1.73 0.05
-
 alpha-naphthylcaprylate pH 5.0, 37°C, D71I mutant enzyme Aspergillus awamori
3.1.1.73 0.079
-
 alpha-naphthylcaprylate pH 5.0, 37°C, wild type enzyme Aspergillus awamori
3.1.1.73 0.15
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D77I mutant enzyme Aspergillus awamori
3.1.1.73 0.18
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D77N mutant enzyme Aspergillus awamori
3.1.1.73 0.29
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D71I mutant enzyme Aspergillus awamori
3.1.1.73 0.33
-
 alpha-naphthylbutyrate pH 5.0, 37°C, wild type enzyme Aspergillus awamori
3.1.1.73 0.63
-
 alpha-naphthylbutyrate pH 5.0, 37°C, T72R mutant enzyme Aspergillus awamori
3.1.1.73 0.63
-
 alpha-naphthylbutyrate pH 5.0, 37°C, Y80F mutant enzyme Aspergillus awamori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Aspergillus awamori
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl polysaccharide + H2O Aspergillus awamori involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O Aspergillus awamori IFO 4033 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus awamori Q9P979 feruloyl esterase type A
-
3.1.1.73 Aspergillus awamori IFO 4033 Q9P979 feruloyl esterase type A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 1-naphthyl butyrate + H2O
-
 Aspergillus awamori 1-naphthol + butanoate
-
 ?
3.1.1.73 1-naphthyl butyrate + H2O
-
 Aspergillus awamori IFO 4033 1-naphthol + butanoate
-
 ?
3.1.1.73 1-naphthyl caprylate + H2O
-
 Aspergillus awamori caprylic acid + 1-naphthol
-
 ?
3.1.1.73 feruloyl arabinoxylan + H2O
-
 Aspergillus awamori ferulic acid + arabinoxylan
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus awamori ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Aspergillus awamori IFO 4033 ferulic acid + ?
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Aspergillus awamori caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Aspergillus awamori IFO 4033 caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus awamori ferulic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Aspergillus awamori p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Aspergillus awamori sinapic acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Aspergillus awamori IFO 4033 sinapic acid + methanol
-
 ?
3.1.1.73 additional information active on alpha-naphthyl esters with chain length from C2 to C8, highest activity towards C4 ester Aspergillus awamori ?
-
 ?
3.1.1.73 additional information active on alpha-naphthyl esters with chain length from C2 to C8, highest activity towards C4 ester Aspergillus awamori IFO 4033 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 0.32
-
 alpha-naphthylcaprylate pH 5.0, 37°C, T72R mutant enzyme Aspergillus awamori
3.1.1.73 0.64
-
 alpha-naphthylcaprylate pH 5.0, 37°C, D71I mutant enzyme Aspergillus awamori
3.1.1.73 0.85
-
 alpha-naphthylcaprylate pH 5.0, 37°C, wild type enzyme Aspergillus awamori
3.1.1.73 1.09
-
 alpha-naphthylcaprylate pH 5.0, 37°C, Y80F mutant enzyme Aspergillus awamori
3.1.1.73 2.33
-
 alpha-naphthylbutyrate pH 5.0, 37°C, wild type enzyme Aspergillus awamori
3.1.1.73 2.7
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D71I mutant enzyme Aspergillus awamori
3.1.1.73 3.38
-
 alpha-naphthylbutyrate pH 5.0, 37°C, Y80F mutant enzyme Aspergillus awamori
3.1.1.73 3.72
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D77I mutant enzyme Aspergillus awamori
3.1.1.73 3.77
-
 alpha-naphthylbutyrate pH 5.0, 37°C, T72R mutant enzyme Aspergillus awamori
3.1.1.73 32.1
-
 alpha-naphthylbutyrate pH 5.0, 37°C, D77N mutant enzyme Aspergillus awamori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
 wild type, D71I, and T72R mutant enzymes Aspergillus awamori
3.1.1.73 6
-
 Y80F mutant enzyme Aspergillus awamori
3.1.1.73 7 8 D77I mutant enzyme Aspergillus awamori
3.1.1.73 7
-
 D77N mutant enzyme Aspergillus awamori