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Literature summary extracted from

  • Anderson, M.A.; Cleland, W.W.; Huang, D.T.; Chan, C.; Shojaei, M.; Christopherson, R.I.
    13C and 15N isotope effects for conversion of L-dihydroorotate to N-carbamyl-L-aspartate using dihydroorotase from hamster and Bacillus caldolyticus (2006), Biochemistry, 45, 7132-7139.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.3 expressed in Escherichia coli strain SO1263 Mesocricetus auratus
3.5.2.3 expressed in Escherichia coli strain SO1263 [Bacillus] caldolyticus
3.5.2.3 overexpression in Escherichia coli strain BL21(DE3) [Bacillus] caldolyticus
3.5.2.3 overexpression in Escherichia coli strain SØ1263/pyrC- Mesocricetus auratus

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.3 Mesocricetus auratus
-
-
-
3.5.2.3 [Bacillus] caldolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.3
-
 Mesocricetus auratus
3.5.2.3
-
 [Bacillus] caldolyticus
3.5.2.3 more than 95% purified by PorosHQ column [Bacillus] caldolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.3 (S)-dihydroorotate + H2O
-
 Mesocricetus auratus N-carbamoyl-L-aspartate
-
 r
3.5.2.3 (S)-dihydroorotate + H2O
-
 [Bacillus] caldolyticus N-carbamoyl-L-aspartate
-
 r
3.5.2.3 N-carbamoyl-L-aspartate
-
 Mesocricetus auratus L-dihydroorotate + H2O
-
 r
3.5.2.3 N-carbamoyl-L-aspartate
-
 [Bacillus] caldolyticus L-dihydroorotate + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
3.5.2.3 BcDHOase
-
 [Bacillus] caldolyticus
3.5.2.3 DHOase
-
 Mesocricetus auratus
3.5.2.3 DHOase
-
 [Bacillus] caldolyticus
3.5.2.3 hDHOase
-
 Mesocricetus auratus
3.5.2.3 pyrC
-
 [Bacillus] caldolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.3 25 45
-
 Mesocricetus auratus
3.5.2.3 25 45
-
 [Bacillus] caldolyticus
3.5.2.3 25 45 differences in kinetic isotope effects due to temperature change are nonexistent for hDHOase even though both systems are run at suboptimal temperatures Mesocricetus auratus
3.5.2.3 37 60
-
 Mesocricetus auratus
3.5.2.3 37 60
-
 [Bacillus] caldolyticus
3.5.2.3 37 60 kinetic isotope effects due to temperature change show a very moderate difference in the secondary kinetic isotope effect for BcDHOase even though both systems are run at suboptimal temperatures [Bacillus] caldolyticus